Study on the relationship of cPLA2, CK-MB, and membrane phospholipid content in acute myocardial infarction

Increased plasma creatine kinase-myoglobin (CK-MB) occurs in patients with acute myocardial infarction (AMI), but its underlying relationship with cytosolic phospholipase A2 (cPLA2) is poorly understood. In the present study we sought to determine cPLA2 activation and its relationship with plasma an...

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Bibliographic Details
Published in:Heart and vessels 2011, Vol.26 (1), p.64-68
Main Authors: Wei-hua, Li, Jun-yu, Han, Chang-qing, Sun, Yong-jun, Guo, Qiang, Xie, Kai-min, Lin, Rong, Wu, Yan, Zhao, Lin, Lin
Format: Article
Language:English
Subjects:
Animals
Biomedical Engineering and Bioengineering
Cardiac Surgery
Cardiology
Creatine Kinase, MB Form - blood
Creatine Kinase, MB Form - metabolism
Disease Models, Animal
Enzymes
Gene expression
Gene Expression Regulation, Enzymologic
Group IV Phospholipases A2 - genetics
Group IV Phospholipases A2 - metabolism
Heart attacks
Immunohistochemistry
Kinases
Lipids
Male
Medicine
Medicine & Public Health
Membrane Lipids - metabolism
Membranes
Myocardial Infarction - enzymology
Myocardial Infarction - genetics
Myocardium - enzymology
Original Article
Phospholipids - metabolism
Proteins
Rats
Rats, Sprague-Dawley
Reverse Transcriptase Polymerase Chain Reaction
RNA, Messenger - metabolism
Time Factors
Vascular Surgery
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Summary:Increased plasma creatine kinase-myoglobin (CK-MB) occurs in patients with acute myocardial infarction (AMI), but its underlying relationship with cytosolic phospholipase A2 (cPLA2) is poorly understood. In the present study we sought to determine cPLA2 activation and its relationship with plasma and myocardial CK-MB level and membrane phospholipids (PLs) in an animal model of AMI. AMI was induced in 60 male Sprague-Dawley rats by ligating the left anterior descending of coronary artery. Rats were randomized into six groups at 0 (sham group), 1, 2, 4, 6, and 12 h after AMI onset (ten rats in each group). cPLA2 was detected by reverse transcription polymerase chain reaction and immunohistochemical staining for mRNA and protein expression, respectively. Plasma and myocardial CK-MB activity were measured by inhibition kinetics, and membrane PLs were determined by phosphorus quantitative method. Myocardial cPLA2 expression increased from 1 h and reached a peak at 2 h after AMI onset ( p  
ISSN:0910-8327
1615-2573
DOI:10.1007/s00380-010-0031-2