Differential affinity of vitronectin versus collagen for synthetic biodegradable scaffolds for urethroplastic applications

Abstract Cell-seeded synthetic polymer scaffolds constitute an emerging technology for urethroplastic applications. The study goal was to identify urethral proteins appropriate for cell attachment and optimize their adsorption onto two types of scaffolds: porous poly(ester urethane) with a poly(capr...

Full description

Saved in:
Bibliographic Details
Published in:Biomaterials 2011-01, Vol.32 (3), p.797-807
Main Authors: Hicks, Bryson G, Lopez, Erasmo A, Eastman, Rocky, Simonovsky, Felix I, Ratner, Buddy D, Wessells, Hunter, Voelzke, Bryan B, Bassuk, James A
Format: Article
Language:English
Subjects:
Adult
Advanced Basic Science
Aged
Biocompatible Materials - chemistry
Collagen - chemistry
Collagen Type I - chemistry
Dentistry
Enzyme-Linked Immunosorbent Assay
Humans
In Vitro Techniques
Microscopy, Electron, Scanning
Middle Aged
Poly-96L/4D-lactide
Polyesters - chemistry
Polyurethanes - chemistry
Porosity
Porous polyurethane
Saturation
Tissue Scaffolds - chemistry
Type I collagen
Type IV collagen
Urethra
Vitronectin
Vitronectin - chemistry
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Abstract Cell-seeded synthetic polymer scaffolds constitute an emerging technology for urethroplastic applications. The study goal was to identify urethral proteins appropriate for cell attachment and optimize their adsorption onto two types of scaffolds: porous poly(ester urethane) with a poly(caprolactone) soft segment (PEU-PCL) and poly-(96% L/4% D)-lactic acid (P96L/4DLA). Specimens from eight men undergoing urethral reconstruction for stricture diseases were subjected to immunohistochemical analysis. Type I collagen, type IV collagen and vitronectin were detected at the interface between the epithelium and its basement membrane. Electrophoresis confirmed that polypeptide chains in the starting material were also present in fractions eluted from adsorbed scaffolds. Over a 4 week incubation assay, only vitronectin exhibited 100% retention levels for all scaffolds. The saturation point for each protein on each scaffold type was determined by titration and ELISA. The collective evidence indicates the concept that vitronectin > type IV collagen > type I collagen are preferred adsorption proteins for PEU-PCL and P96L/4DLA.
ISSN:0142-9612
1878-5905
DOI:10.1016/j.biomaterials.2010.09.050