Loading…
Pulsed field, PCR ribotyping and multiplex PCR analysis of Yersinia enterocolitica strains isolated from meat food in San Luis Argentina
The characterization of phenotypic and genotypic virulence markers of Yersinia enterocolitica strains belonging to biotypes (B) 1A, 2 and 3, mostly isolated from food in San Luis, Argentina, and the assessment of their genotypic diversity using PFGE and PCR ribotyping, were performed in our laborato...
Saved in:
| Published in: | Food microbiology 2011-02, Vol.28 (1), p.21-28 |
|---|---|
| Main Authors: | , , , , , |
| Format: | Article |
| Language: | English |
| Subjects: | |
| Citations: | Items that cite this one |
| Online Access: | Get full text |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| cited_by | cdi_FETCH-LOGICAL-c435t-f99bf072fa790032508b052d2f4e1cf624f34e260b4c9c86f1cdccfc157f43863 |
|---|---|
| cites | |
| container_end_page | 28 |
| container_issue | 1 |
| container_start_page | 21 |
| container_title | Food microbiology |
| container_volume | 28 |
| creator | Lucero Estrada, Cecilia S.M. Velázquez, Lidia del Carmen Escudero, María Esther Favier, Gabriela Isabel Lazarte, Valeria Stefanini de Guzmán, Ana María |
| description | The characterization of phenotypic and genotypic virulence markers of
Yersinia enterocolitica strains belonging to biotypes (B) 1A, 2 and 3, mostly isolated from food in San Luis, Argentina, and the assessment of their genotypic diversity using PFGE and PCR ribotyping, were performed in our laboratory for the first time. Thirty five
Y. enterocolitica strains, two reference strains and 33 strains isolated in our laboratory were studied. The presence of
virF,
ail,
ystA, and
myfA genes was investigated by multiplex PCR. The pathogenic potential of B1A strains, the most predominant biotype of
Y. enterocolitica strains isolated from meat in our region, was investigated by simple PCR. Four B1A strains were positive for
ystB gene. Four
Y. enterocolitica 2/O:9 (bio/serotype) and two 3/O:5 strains isolated in our laboratory showed virulence-related results in the phenotypic tests and multiplex PCR. A good correlation between the expression of virulence markers and their corresponding genotypes was observed for most strains. Sixteen genomic types (GT) and 9 different intergenic spacer region (SR) groups were generated by PFGE and PCR ribotyping, respectively. In both cases the
Y. enterocolitica 2/O:9 strains were separately clustered from 1A and 3/O:5 strains. Meat foods might be vehicles of transmission of pathogenic
Y. enterocolitica strains in our region. |
| doi_str_mv | 10.1016/j.fm.2010.07.026 |
| format | article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_855683503</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S074000201000211X</els_id><sourcerecordid>855683503</sourcerecordid><originalsourceid>FETCH-LOGICAL-c435t-f99bf072fa790032508b052d2f4e1cf624f34e260b4c9c86f1cdccfc157f43863</originalsourceid><addsrcrecordid>eNqFkUuP0zAUhS0EYjqFPSvwBs2GlOtXnMxuVPGSKjFimAUry3HsylViFztB9B_ws3FpgRViZV35O-ce3YPQMwIrAqR-vVu5cUWhjCBXQOsHaEGgFVXbts1DtADJoQKgcIEuc94BECJY-xhdUAKilpIs0I_beci2x87boX-Fb9efcPJdnA57H7ZYhx6P8zD5_WC___rUQQ-H7DOODn-xKfvgNbZhsimaOPjJG43zlLQPGfscBz0dzVMc8Wj1hF2MPfYB3-mAN3OxuUnbovZBP0GPnC5Rnp7fJbp_--bz-n21-fjuw_pmUxnOxFS5tu0cSOq0bAEYFdB0IGhPHbfEuJpyx7ilNXTctKapHTG9Mc4QIR1nTc2W6Orku0_x62zzpEafjR0GHWycs2qEqBsmgP2XlDUjtZD86Akn0qSYc7JO7ZMfdTooAupYlNopN6pjUQqkKkUVyfOz-dyNtv8j-N1MAV6eAZ2NHlzSwfj8l2Oc0abEXKIXJ87pqPQ2Feb-rmzipW3JW5CFuD4Rtpz1m7dJZeNtMLb3yZpJ9dH_O-dPav25bw</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>763165746</pqid></control><display><type>article</type><title>Pulsed field, PCR ribotyping and multiplex PCR analysis of Yersinia enterocolitica strains isolated from meat food in San Luis Argentina</title><source>ScienceDirect Freedom Collection</source><creator>Lucero Estrada, Cecilia S.M. ; Velázquez, Lidia del Carmen ; Escudero, María Esther ; Favier, Gabriela Isabel ; Lazarte, Valeria ; Stefanini de Guzmán, Ana María</creator><creatorcontrib>Lucero Estrada, Cecilia S.M. ; Velázquez, Lidia del Carmen ; Escudero, María Esther ; Favier, Gabriela Isabel ; Lazarte, Valeria ; Stefanini de Guzmán, Ana María</creatorcontrib><description>The characterization of phenotypic and genotypic virulence markers of
Yersinia enterocolitica strains belonging to biotypes (B) 1A, 2 and 3, mostly isolated from food in San Luis, Argentina, and the assessment of their genotypic diversity using PFGE and PCR ribotyping, were performed in our laboratory for the first time. Thirty five
Y. enterocolitica strains, two reference strains and 33 strains isolated in our laboratory were studied. The presence of
virF,
ail,
ystA, and
myfA genes was investigated by multiplex PCR. The pathogenic potential of B1A strains, the most predominant biotype of
Y. enterocolitica strains isolated from meat in our region, was investigated by simple PCR. Four B1A strains were positive for
ystB gene. Four
Y. enterocolitica 2/O:9 (bio/serotype) and two 3/O:5 strains isolated in our laboratory showed virulence-related results in the phenotypic tests and multiplex PCR. A good correlation between the expression of virulence markers and their corresponding genotypes was observed for most strains. Sixteen genomic types (GT) and 9 different intergenic spacer region (SR) groups were generated by PFGE and PCR ribotyping, respectively. In both cases the
Y. enterocolitica 2/O:9 strains were separately clustered from 1A and 3/O:5 strains. Meat foods might be vehicles of transmission of pathogenic
Y. enterocolitica strains in our region.</description><identifier>ISSN: 0740-0020</identifier><identifier>EISSN: 1095-9998</identifier><identifier>DOI: 10.1016/j.fm.2010.07.026</identifier><identifier>PMID: 21056771</identifier><identifier>CODEN: FOMIE5</identifier><language>eng</language><publisher>Kidlington: Elsevier Ltd</publisher><subject>Argentina ; Biological and medical sciences ; biotypes ; Electrophoresis, Gel, Pulsed-Field - methods ; Food industries ; Food Microbiology ; Fundamental and applied biological sciences. Psychology ; genes ; Genes, Bacterial ; Genotype ; intergenic DNA ; meat ; Meat and meat product industries ; Meat Products - microbiology ; Molecular characterization ; Multiplex PCR ; PCR ribotyping ; PFGE ; Phenotype ; polymerase chain reaction ; Polymerase Chain Reaction - methods ; Ribotyping - methods ; serotypes ; virulence ; Yersinia enterocolitica ; Yersinia enterocolitica - classification ; Yersinia enterocolitica - genetics ; Yersinia enterocolitica - isolation & purification ; Yersinia enterocolitica - pathogenicity</subject><ispartof>Food microbiology, 2011-02, Vol.28 (1), p.21-28</ispartof><rights>2010 Elsevier Ltd</rights><rights>2015 INIST-CNRS</rights><rights>Copyright © 2010 Elsevier Ltd. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c435t-f99bf072fa790032508b052d2f4e1cf624f34e260b4c9c86f1cdccfc157f43863</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27923,27924</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=23432850$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21056771$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lucero Estrada, Cecilia S.M.</creatorcontrib><creatorcontrib>Velázquez, Lidia del Carmen</creatorcontrib><creatorcontrib>Escudero, María Esther</creatorcontrib><creatorcontrib>Favier, Gabriela Isabel</creatorcontrib><creatorcontrib>Lazarte, Valeria</creatorcontrib><creatorcontrib>Stefanini de Guzmán, Ana María</creatorcontrib><title>Pulsed field, PCR ribotyping and multiplex PCR analysis of Yersinia enterocolitica strains isolated from meat food in San Luis Argentina</title><title>Food microbiology</title><addtitle>Food Microbiol</addtitle><description>The characterization of phenotypic and genotypic virulence markers of
Yersinia enterocolitica strains belonging to biotypes (B) 1A, 2 and 3, mostly isolated from food in San Luis, Argentina, and the assessment of their genotypic diversity using PFGE and PCR ribotyping, were performed in our laboratory for the first time. Thirty five
Y. enterocolitica strains, two reference strains and 33 strains isolated in our laboratory were studied. The presence of
virF,
ail,
ystA, and
myfA genes was investigated by multiplex PCR. The pathogenic potential of B1A strains, the most predominant biotype of
Y. enterocolitica strains isolated from meat in our region, was investigated by simple PCR. Four B1A strains were positive for
ystB gene. Four
Y. enterocolitica 2/O:9 (bio/serotype) and two 3/O:5 strains isolated in our laboratory showed virulence-related results in the phenotypic tests and multiplex PCR. A good correlation between the expression of virulence markers and their corresponding genotypes was observed for most strains. Sixteen genomic types (GT) and 9 different intergenic spacer region (SR) groups were generated by PFGE and PCR ribotyping, respectively. In both cases the
Y. enterocolitica 2/O:9 strains were separately clustered from 1A and 3/O:5 strains. Meat foods might be vehicles of transmission of pathogenic
Y. enterocolitica strains in our region.</description><subject>Argentina</subject><subject>Biological and medical sciences</subject><subject>biotypes</subject><subject>Electrophoresis, Gel, Pulsed-Field - methods</subject><subject>Food industries</subject><subject>Food Microbiology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>genes</subject><subject>Genes, Bacterial</subject><subject>Genotype</subject><subject>intergenic DNA</subject><subject>meat</subject><subject>Meat and meat product industries</subject><subject>Meat Products - microbiology</subject><subject>Molecular characterization</subject><subject>Multiplex PCR</subject><subject>PCR ribotyping</subject><subject>PFGE</subject><subject>Phenotype</subject><subject>polymerase chain reaction</subject><subject>Polymerase Chain Reaction - methods</subject><subject>Ribotyping - methods</subject><subject>serotypes</subject><subject>virulence</subject><subject>Yersinia enterocolitica</subject><subject>Yersinia enterocolitica - classification</subject><subject>Yersinia enterocolitica - genetics</subject><subject>Yersinia enterocolitica - isolation & purification</subject><subject>Yersinia enterocolitica - pathogenicity</subject><issn>0740-0020</issn><issn>1095-9998</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><recordid>eNqFkUuP0zAUhS0EYjqFPSvwBs2GlOtXnMxuVPGSKjFimAUry3HsylViFztB9B_ws3FpgRViZV35O-ce3YPQMwIrAqR-vVu5cUWhjCBXQOsHaEGgFVXbts1DtADJoQKgcIEuc94BECJY-xhdUAKilpIs0I_beci2x87boX-Fb9efcPJdnA57H7ZYhx6P8zD5_WC___rUQQ-H7DOODn-xKfvgNbZhsimaOPjJG43zlLQPGfscBz0dzVMc8Wj1hF2MPfYB3-mAN3OxuUnbovZBP0GPnC5Rnp7fJbp_--bz-n21-fjuw_pmUxnOxFS5tu0cSOq0bAEYFdB0IGhPHbfEuJpyx7ilNXTctKapHTG9Mc4QIR1nTc2W6Orku0_x62zzpEafjR0GHWycs2qEqBsmgP2XlDUjtZD86Akn0qSYc7JO7ZMfdTooAupYlNopN6pjUQqkKkUVyfOz-dyNtv8j-N1MAV6eAZ2NHlzSwfj8l2Oc0abEXKIXJ87pqPQ2Feb-rmzipW3JW5CFuD4Rtpz1m7dJZeNtMLb3yZpJ9dH_O-dPav25bw</recordid><startdate>20110201</startdate><enddate>20110201</enddate><creator>Lucero Estrada, Cecilia S.M.</creator><creator>Velázquez, Lidia del Carmen</creator><creator>Escudero, María Esther</creator><creator>Favier, Gabriela Isabel</creator><creator>Lazarte, Valeria</creator><creator>Stefanini de Guzmán, Ana María</creator><general>Elsevier Ltd</general><general>Elsevier</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7QL</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope></search><sort><creationdate>20110201</creationdate><title>Pulsed field, PCR ribotyping and multiplex PCR analysis of Yersinia enterocolitica strains isolated from meat food in San Luis Argentina</title><author>Lucero Estrada, Cecilia S.M. ; Velázquez, Lidia del Carmen ; Escudero, María Esther ; Favier, Gabriela Isabel ; Lazarte, Valeria ; Stefanini de Guzmán, Ana María</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c435t-f99bf072fa790032508b052d2f4e1cf624f34e260b4c9c86f1cdccfc157f43863</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Argentina</topic><topic>Biological and medical sciences</topic><topic>biotypes</topic><topic>Electrophoresis, Gel, Pulsed-Field - methods</topic><topic>Food industries</topic><topic>Food Microbiology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>genes</topic><topic>Genes, Bacterial</topic><topic>Genotype</topic><topic>intergenic DNA</topic><topic>meat</topic><topic>Meat and meat product industries</topic><topic>Meat Products - microbiology</topic><topic>Molecular characterization</topic><topic>Multiplex PCR</topic><topic>PCR ribotyping</topic><topic>PFGE</topic><topic>Phenotype</topic><topic>polymerase chain reaction</topic><topic>Polymerase Chain Reaction - methods</topic><topic>Ribotyping - methods</topic><topic>serotypes</topic><topic>virulence</topic><topic>Yersinia enterocolitica</topic><topic>Yersinia enterocolitica - classification</topic><topic>Yersinia enterocolitica - genetics</topic><topic>Yersinia enterocolitica - isolation & purification</topic><topic>Yersinia enterocolitica - pathogenicity</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lucero Estrada, Cecilia S.M.</creatorcontrib><creatorcontrib>Velázquez, Lidia del Carmen</creatorcontrib><creatorcontrib>Escudero, María Esther</creatorcontrib><creatorcontrib>Favier, Gabriela Isabel</creatorcontrib><creatorcontrib>Lazarte, Valeria</creatorcontrib><creatorcontrib>Stefanini de Guzmán, Ana María</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Food microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lucero Estrada, Cecilia S.M.</au><au>Velázquez, Lidia del Carmen</au><au>Escudero, María Esther</au><au>Favier, Gabriela Isabel</au><au>Lazarte, Valeria</au><au>Stefanini de Guzmán, Ana María</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Pulsed field, PCR ribotyping and multiplex PCR analysis of Yersinia enterocolitica strains isolated from meat food in San Luis Argentina</atitle><jtitle>Food microbiology</jtitle><addtitle>Food Microbiol</addtitle><date>2011-02-01</date><risdate>2011</risdate><volume>28</volume><issue>1</issue><spage>21</spage><epage>28</epage><pages>21-28</pages><issn>0740-0020</issn><eissn>1095-9998</eissn><coden>FOMIE5</coden><abstract>The characterization of phenotypic and genotypic virulence markers of
Yersinia enterocolitica strains belonging to biotypes (B) 1A, 2 and 3, mostly isolated from food in San Luis, Argentina, and the assessment of their genotypic diversity using PFGE and PCR ribotyping, were performed in our laboratory for the first time. Thirty five
Y. enterocolitica strains, two reference strains and 33 strains isolated in our laboratory were studied. The presence of
virF,
ail,
ystA, and
myfA genes was investigated by multiplex PCR. The pathogenic potential of B1A strains, the most predominant biotype of
Y. enterocolitica strains isolated from meat in our region, was investigated by simple PCR. Four B1A strains were positive for
ystB gene. Four
Y. enterocolitica 2/O:9 (bio/serotype) and two 3/O:5 strains isolated in our laboratory showed virulence-related results in the phenotypic tests and multiplex PCR. A good correlation between the expression of virulence markers and their corresponding genotypes was observed for most strains. Sixteen genomic types (GT) and 9 different intergenic spacer region (SR) groups were generated by PFGE and PCR ribotyping, respectively. In both cases the
Y. enterocolitica 2/O:9 strains were separately clustered from 1A and 3/O:5 strains. Meat foods might be vehicles of transmission of pathogenic
Y. enterocolitica strains in our region.</abstract><cop>Kidlington</cop><pub>Elsevier Ltd</pub><pmid>21056771</pmid><doi>10.1016/j.fm.2010.07.026</doi><tpages>8</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0740-0020 |
| ispartof | Food microbiology, 2011-02, Vol.28 (1), p.21-28 |
| issn | 0740-0020 1095-9998 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_855683503 |
| source | ScienceDirect Freedom Collection |
| subjects | Argentina Biological and medical sciences biotypes Electrophoresis, Gel, Pulsed-Field - methods Food industries Food Microbiology Fundamental and applied biological sciences. Psychology genes Genes, Bacterial Genotype intergenic DNA meat Meat and meat product industries Meat Products - microbiology Molecular characterization Multiplex PCR PCR ribotyping PFGE Phenotype polymerase chain reaction Polymerase Chain Reaction - methods Ribotyping - methods serotypes virulence Yersinia enterocolitica Yersinia enterocolitica - classification Yersinia enterocolitica - genetics Yersinia enterocolitica - isolation & purification Yersinia enterocolitica - pathogenicity |
| title | Pulsed field, PCR ribotyping and multiplex PCR analysis of Yersinia enterocolitica strains isolated from meat food in San Luis Argentina |
| url | http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-08T13%3A05%3A14IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Pulsed%20field,%20PCR%20ribotyping%20and%20multiplex%20PCR%20analysis%20of%20Yersinia%20enterocolitica%20strains%20isolated%20from%20meat%20food%20in%20San%20Luis%20Argentina&rft.jtitle=Food%20microbiology&rft.au=Lucero%20Estrada,%20Cecilia%20S.M.&rft.date=2011-02-01&rft.volume=28&rft.issue=1&rft.spage=21&rft.epage=28&rft.pages=21-28&rft.issn=0740-0020&rft.eissn=1095-9998&rft.coden=FOMIE5&rft_id=info:doi/10.1016/j.fm.2010.07.026&rft_dat=%3Cproquest_cross%3E855683503%3C/proquest_cross%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c435t-f99bf072fa790032508b052d2f4e1cf624f34e260b4c9c86f1cdccfc157f43863%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=763165746&rft_id=info:pmid/21056771&rfr_iscdi=true |