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Surfactant enhanced lipase containing films characterized by confocal laser scanning microscopy
Confocal laser scanning microscopy (CLSM) in combination with a fluorescently labeling enzyme dye, LavaPurple™, was demonstrated as a technique for the visualization of Thermomyces ( Humicola) lanuginosa lipase (LIP HLL) and Candida antarctica lipase A (LIP CA) within a transparent latex coating. Ad...
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| Published in: | Colloids and surfaces, B, Biointerfaces B, Biointerfaces, 2011-02, Vol.82 (2), p.291-296 |
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| cites | cdi_FETCH-LOGICAL-c456t-13255556a4d4fdfeb05adc52a31c7569063ec6555d7531ea5f22e458ab6fe4783 |
| container_end_page | 296 |
| container_issue | 2 |
| container_start_page | 291 |
| container_title | Colloids and surfaces, B, Biointerfaces |
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| creator | Jayawardena, Menuk B. Yee, Lachlan H. Rainbow, Ian J. Bergquist, Peter Such, Christopher Steinberg, Peter D. Kjelleberg, Staffan J. |
| description | Confocal laser scanning microscopy (CLSM) in combination with a fluorescently labeling enzyme dye, LavaPurple™, was demonstrated as a technique for the visualization of
Thermomyces (
Humicola)
lanuginosa lipase (LIP
HLL) and
Candida antarctica lipase A (LIP
CA) within a transparent latex coating. Addition of Teric Surfactants (C
16 non-ionic Teric 475, 1.8% (w/w) or C
10 non-ionic Teric 460, 2.0% (w/w)) significantly increased the accumulation of both LIP
HLL and LIP
CA to the surface of a latex coating. An α-naphthyl acetate substrate assay was used to quantify the accumulated lipase. The results derived from the acetate assay correlated with the enzyme accumulation (at the surface) observed in the CLSM images of the latex coating. This correlation demonstrated that the increased enzyme accumulation within the top 2
μm of the latex film was responsible for the increase in surface enzymatic activity. The combination of CLSM imagery and quantifiable image analysis provided a valuable tool for the optimization of surfactant concentrations for maximizing the activity of an enzyme (and potentially other additives) within a latex coating. |
| doi_str_mv | 10.1016/j.colsurfb.2010.08.042 |
| format | article |
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Thermomyces (
Humicola)
lanuginosa lipase (LIP
HLL) and
Candida antarctica lipase A (LIP
CA) within a transparent latex coating. Addition of Teric Surfactants (C
16 non-ionic Teric 475, 1.8% (w/w) or C
10 non-ionic Teric 460, 2.0% (w/w)) significantly increased the accumulation of both LIP
HLL and LIP
CA to the surface of a latex coating. An α-naphthyl acetate substrate assay was used to quantify the accumulated lipase. The results derived from the acetate assay correlated with the enzyme accumulation (at the surface) observed in the CLSM images of the latex coating. This correlation demonstrated that the increased enzyme accumulation within the top 2
μm of the latex film was responsible for the increase in surface enzymatic activity. The combination of CLSM imagery and quantifiable image analysis provided a valuable tool for the optimization of surfactant concentrations for maximizing the activity of an enzyme (and potentially other additives) within a latex coating.</description><identifier>ISSN: 0927-7765</identifier><identifier>EISSN: 1873-4367</identifier><identifier>DOI: 10.1016/j.colsurfb.2010.08.042</identifier><identifier>PMID: 21041068</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Acetates ; additives ; Ascomycota - enzymology ; assays ; Candida - enzymology ; Candida antarctica ; Catalysis ; Catalytic coating ; Coating ; coatings ; Confocal ; Confocal laser scanning microscopy ; Correlation ; enzyme activity ; Enzymes ; films (materials) ; fluorescent dyes ; fluorescent labeling ; Humicola ; Humicola lanuginosa ; image analysis ; Latex ; Latex - chemistry ; Lipase ; Lipase - chemistry ; Materials Testing ; Microscopy, Confocal - methods ; Pseudozyma antarctica ; Scanning microscopy ; Software ; Surface enzyme activity ; Surface Properties ; Surface-Active Agents - chemistry ; surfaces ; Surfactant ; Surfactants ; Thermomyces lanuginosus ; triacylglycerol lipase</subject><ispartof>Colloids and surfaces, B, Biointerfaces, 2011-02, Vol.82 (2), p.291-296</ispartof><rights>2010 Elsevier B.V.</rights><rights>Copyright © 2010 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c456t-13255556a4d4fdfeb05adc52a31c7569063ec6555d7531ea5f22e458ab6fe4783</citedby><cites>FETCH-LOGICAL-c456t-13255556a4d4fdfeb05adc52a31c7569063ec6555d7531ea5f22e458ab6fe4783</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21041068$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Jayawardena, Menuk B.</creatorcontrib><creatorcontrib>Yee, Lachlan H.</creatorcontrib><creatorcontrib>Rainbow, Ian J.</creatorcontrib><creatorcontrib>Bergquist, Peter</creatorcontrib><creatorcontrib>Such, Christopher</creatorcontrib><creatorcontrib>Steinberg, Peter D.</creatorcontrib><creatorcontrib>Kjelleberg, Staffan J.</creatorcontrib><title>Surfactant enhanced lipase containing films characterized by confocal laser scanning microscopy</title><title>Colloids and surfaces, B, Biointerfaces</title><addtitle>Colloids Surf B Biointerfaces</addtitle><description>Confocal laser scanning microscopy (CLSM) in combination with a fluorescently labeling enzyme dye, LavaPurple™, was demonstrated as a technique for the visualization of
Thermomyces (
Humicola)
lanuginosa lipase (LIP
HLL) and
Candida antarctica lipase A (LIP
CA) within a transparent latex coating. Addition of Teric Surfactants (C
16 non-ionic Teric 475, 1.8% (w/w) or C
10 non-ionic Teric 460, 2.0% (w/w)) significantly increased the accumulation of both LIP
HLL and LIP
CA to the surface of a latex coating. An α-naphthyl acetate substrate assay was used to quantify the accumulated lipase. The results derived from the acetate assay correlated with the enzyme accumulation (at the surface) observed in the CLSM images of the latex coating. This correlation demonstrated that the increased enzyme accumulation within the top 2
μm of the latex film was responsible for the increase in surface enzymatic activity. The combination of CLSM imagery and quantifiable image analysis provided a valuable tool for the optimization of surfactant concentrations for maximizing the activity of an enzyme (and potentially other additives) within a latex coating.</description><subject>Acetates</subject><subject>additives</subject><subject>Ascomycota - enzymology</subject><subject>assays</subject><subject>Candida - enzymology</subject><subject>Candida antarctica</subject><subject>Catalysis</subject><subject>Catalytic coating</subject><subject>Coating</subject><subject>coatings</subject><subject>Confocal</subject><subject>Confocal laser scanning microscopy</subject><subject>Correlation</subject><subject>enzyme activity</subject><subject>Enzymes</subject><subject>films (materials)</subject><subject>fluorescent dyes</subject><subject>fluorescent labeling</subject><subject>Humicola</subject><subject>Humicola lanuginosa</subject><subject>image analysis</subject><subject>Latex</subject><subject>Latex - chemistry</subject><subject>Lipase</subject><subject>Lipase - chemistry</subject><subject>Materials Testing</subject><subject>Microscopy, Confocal - methods</subject><subject>Pseudozyma antarctica</subject><subject>Scanning microscopy</subject><subject>Software</subject><subject>Surface enzyme activity</subject><subject>Surface Properties</subject><subject>Surface-Active Agents - chemistry</subject><subject>surfaces</subject><subject>Surfactant</subject><subject>Surfactants</subject><subject>Thermomyces lanuginosus</subject><subject>triacylglycerol lipase</subject><issn>0927-7765</issn><issn>1873-4367</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><recordid>eNqF0ctu1DAUBmCroqJD4RXa7GCTqe_27EAVN6kSi9K1deIctx4l8WBnKg1Pj8O0LKk3lqzPvy8_IReMrhll-mq79mko-xy6Nad1kdo1lfyErJg1opVCm1dkRTfctMZodUbelLKllHLJzGtyxhmVjGq7Iu62ZoCfYZobnB5g8tg3Q9xBwcanaYY4xem-CXEYS-MfIFeLOf6uqjssIiQPQzNUn5viYfrLx-hzKj7tDm_JaYCh4Lun-Zzcffn88_pbe_Pj6_frTzetl0rPLRNc1aFB9jL0ATuqoPeKg2DeKL2hWqDXVfRGCYagAucolYVOB5TGinPy_pi7y-nXHsvsxlg8DgNMmPbF2RpurZT0ZcmUksoIXeWH_0pmDBVV2k2l-kiXd5eMwe1yHCEfHKNuKcxt3XNhbinMUetqYXXjxdMZ-27E_t-254YquDyCAMnBfY7F3d3WBFXb1IbaJeLjUWD938eI2RUfcSkyZvSz61N86RZ_ALjLtFw</recordid><startdate>20110201</startdate><enddate>20110201</enddate><creator>Jayawardena, Menuk B.</creator><creator>Yee, Lachlan H.</creator><creator>Rainbow, Ian J.</creator><creator>Bergquist, Peter</creator><creator>Such, Christopher</creator><creator>Steinberg, Peter D.</creator><creator>Kjelleberg, Staffan J.</creator><general>Elsevier B.V</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SR</scope><scope>7U5</scope><scope>8BQ</scope><scope>8FD</scope><scope>H8D</scope><scope>JG9</scope><scope>L7M</scope><scope>7X8</scope><scope>7QO</scope><scope>FR3</scope><scope>P64</scope></search><sort><creationdate>20110201</creationdate><title>Surfactant enhanced lipase containing films characterized by confocal laser scanning microscopy</title><author>Jayawardena, Menuk B. ; Yee, Lachlan H. ; Rainbow, Ian J. ; Bergquist, Peter ; Such, Christopher ; Steinberg, Peter D. ; Kjelleberg, Staffan J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c456t-13255556a4d4fdfeb05adc52a31c7569063ec6555d7531ea5f22e458ab6fe4783</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Acetates</topic><topic>additives</topic><topic>Ascomycota - enzymology</topic><topic>assays</topic><topic>Candida - enzymology</topic><topic>Candida antarctica</topic><topic>Catalysis</topic><topic>Catalytic coating</topic><topic>Coating</topic><topic>coatings</topic><topic>Confocal</topic><topic>Confocal laser scanning microscopy</topic><topic>Correlation</topic><topic>enzyme activity</topic><topic>Enzymes</topic><topic>films (materials)</topic><topic>fluorescent dyes</topic><topic>fluorescent labeling</topic><topic>Humicola</topic><topic>Humicola lanuginosa</topic><topic>image analysis</topic><topic>Latex</topic><topic>Latex - chemistry</topic><topic>Lipase</topic><topic>Lipase - chemistry</topic><topic>Materials Testing</topic><topic>Microscopy, Confocal - methods</topic><topic>Pseudozyma antarctica</topic><topic>Scanning microscopy</topic><topic>Software</topic><topic>Surface enzyme activity</topic><topic>Surface Properties</topic><topic>Surface-Active Agents - chemistry</topic><topic>surfaces</topic><topic>Surfactant</topic><topic>Surfactants</topic><topic>Thermomyces lanuginosus</topic><topic>triacylglycerol lipase</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Jayawardena, Menuk B.</creatorcontrib><creatorcontrib>Yee, Lachlan H.</creatorcontrib><creatorcontrib>Rainbow, Ian J.</creatorcontrib><creatorcontrib>Bergquist, Peter</creatorcontrib><creatorcontrib>Such, Christopher</creatorcontrib><creatorcontrib>Steinberg, Peter D.</creatorcontrib><creatorcontrib>Kjelleberg, Staffan J.</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Engineered Materials Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Aerospace Database</collection><collection>Materials Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>MEDLINE - Academic</collection><collection>Biotechnology Research Abstracts</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Colloids and surfaces, B, Biointerfaces</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Jayawardena, Menuk B.</au><au>Yee, Lachlan H.</au><au>Rainbow, Ian J.</au><au>Bergquist, Peter</au><au>Such, Christopher</au><au>Steinberg, Peter D.</au><au>Kjelleberg, Staffan J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Surfactant enhanced lipase containing films characterized by confocal laser scanning microscopy</atitle><jtitle>Colloids and surfaces, B, Biointerfaces</jtitle><addtitle>Colloids Surf B Biointerfaces</addtitle><date>2011-02-01</date><risdate>2011</risdate><volume>82</volume><issue>2</issue><spage>291</spage><epage>296</epage><pages>291-296</pages><issn>0927-7765</issn><eissn>1873-4367</eissn><abstract>Confocal laser scanning microscopy (CLSM) in combination with a fluorescently labeling enzyme dye, LavaPurple™, was demonstrated as a technique for the visualization of
Thermomyces (
Humicola)
lanuginosa lipase (LIP
HLL) and
Candida antarctica lipase A (LIP
CA) within a transparent latex coating. Addition of Teric Surfactants (C
16 non-ionic Teric 475, 1.8% (w/w) or C
10 non-ionic Teric 460, 2.0% (w/w)) significantly increased the accumulation of both LIP
HLL and LIP
CA to the surface of a latex coating. An α-naphthyl acetate substrate assay was used to quantify the accumulated lipase. The results derived from the acetate assay correlated with the enzyme accumulation (at the surface) observed in the CLSM images of the latex coating. This correlation demonstrated that the increased enzyme accumulation within the top 2
μm of the latex film was responsible for the increase in surface enzymatic activity. The combination of CLSM imagery and quantifiable image analysis provided a valuable tool for the optimization of surfactant concentrations for maximizing the activity of an enzyme (and potentially other additives) within a latex coating.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>21041068</pmid><doi>10.1016/j.colsurfb.2010.08.042</doi><tpages>6</tpages></addata></record> |
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| ispartof | Colloids and surfaces, B, Biointerfaces, 2011-02, Vol.82 (2), p.291-296 |
| issn | 0927-7765 1873-4367 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_855688440 |
| source | ScienceDirect Freedom Collection |
| subjects | Acetates additives Ascomycota - enzymology assays Candida - enzymology Candida antarctica Catalysis Catalytic coating Coating coatings Confocal Confocal laser scanning microscopy Correlation enzyme activity Enzymes films (materials) fluorescent dyes fluorescent labeling Humicola Humicola lanuginosa image analysis Latex Latex - chemistry Lipase Lipase - chemistry Materials Testing Microscopy, Confocal - methods Pseudozyma antarctica Scanning microscopy Software Surface enzyme activity Surface Properties Surface-Active Agents - chemistry surfaces Surfactant Surfactants Thermomyces lanuginosus triacylglycerol lipase |
| title | Surfactant enhanced lipase containing films characterized by confocal laser scanning microscopy |
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