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Engineering Bone Formation from Human Dental Pulp- and Periodontal Ligament-Derived Cells
A robust method for inducing bone formation from cultured dental mesenchymal cells has not been established. In this study, a method for generating bone tissue in vivo from cultured human dental pulp- and periodontal ligament-derived cells (DPCs and PDLCs, respectively) was designed using exogenous...
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| Published in: | Annals of biomedical engineering 2011-01, Vol.39 (1), p.26-34 |
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| container_end_page | 34 |
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| container_title | Annals of biomedical engineering |
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| creator | Ikeda, Hideyoshi Sumita, Yoshinori Ikeda, Mihoko Ikeda, Hisazumi Okumura, Teruhito Sakai, Eiko Nishimura, Masahiro Asahina, Izumi |
| description | A robust method for inducing bone formation from cultured dental mesenchymal cells has not been established. In this study, a method for generating bone tissue
in vivo
from cultured human dental pulp- and periodontal ligament-derived cells (DPCs and PDLCs, respectively) was designed using exogenous bone morphogenetic protein 2 (BMP2). DPCs and PDLCs showed enhanced alkaline phosphatase (ALP) activity and calcified nodule formation in medium containing dexamethasone, β-glycerophosphate, and ascorbic acid (osteogenic medium). However, the addition of recombinant human bone morphogenetic protein 2 (rhBMP2) to osteogenic medium remarkably increased ALP activity and
in vitro
calcification above the increases observed with osteogenic medium alone. rhBMP2 also significantly upregulated the expression of
osteocalcin
,
osteopontin
, and
dentin matrix protein 1
mRNA in both cell types cultured in osteogenic medium. Finally, we detected prominent bone-like tissue formation
in vivo
when cells had been exposed to rhBMP2 in osteogenic medium. In contrast, treatments with osteogenic medium or rhBMP2 alone could not induce abundant mineralized tissue formation. We propose here that treatment with rhBMP2 in osteogenic medium can make dental mesenchymal tissues a highly useful source of cells for bone tissue engineering. In addition, both DPCs and PDLCs showed similar and remarkable osteo-inducibility. |
| doi_str_mv | 10.1007/s10439-010-0115-2 |
| format | article |
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in vivo
from cultured human dental pulp- and periodontal ligament-derived cells (DPCs and PDLCs, respectively) was designed using exogenous bone morphogenetic protein 2 (BMP2). DPCs and PDLCs showed enhanced alkaline phosphatase (ALP) activity and calcified nodule formation in medium containing dexamethasone, β-glycerophosphate, and ascorbic acid (osteogenic medium). However, the addition of recombinant human bone morphogenetic protein 2 (rhBMP2) to osteogenic medium remarkably increased ALP activity and
in vitro
calcification above the increases observed with osteogenic medium alone. rhBMP2 also significantly upregulated the expression of
osteocalcin
,
osteopontin
, and
dentin matrix protein 1
mRNA in both cell types cultured in osteogenic medium. Finally, we detected prominent bone-like tissue formation
in vivo
when cells had been exposed to rhBMP2 in osteogenic medium. In contrast, treatments with osteogenic medium or rhBMP2 alone could not induce abundant mineralized tissue formation. We propose here that treatment with rhBMP2 in osteogenic medium can make dental mesenchymal tissues a highly useful source of cells for bone tissue engineering. In addition, both DPCs and PDLCs showed similar and remarkable osteo-inducibility.</description><identifier>ISSN: 0090-6964</identifier><identifier>EISSN: 1573-9686</identifier><identifier>DOI: 10.1007/s10439-010-0115-2</identifier><identifier>PMID: 20614244</identifier><language>eng</language><publisher>Boston: Springer US</publisher><subject>Biochemistry ; Biocompatibility ; Biological and Medical Physics ; Biomedical and Life Sciences ; Biomedical Engineering and Bioengineering ; Biomedical materials ; Biomedicine ; Biophysics ; Bone Development - drug effects ; Bone Development - physiology ; Bone Morphogenetic Protein 2 - administration & dosage ; Bones ; Cell Differentiation ; Cells, Cultured ; Classical Mechanics ; Coculture Techniques ; Dental Pulp - cytology ; Dental Pulp - drug effects ; Dental Pulp - physiology ; Human ; Humans ; In vivo testing ; In vivo tests ; Osteogenesis - genetics ; Osteogenesis - physiology ; Periodontal Ligament - cytology ; Periodontal Ligament - drug effects ; Periodontal Ligament - physiology ; Proteins ; Surgical implants ; Tissue Engineering - methods</subject><ispartof>Annals of biomedical engineering, 2011-01, Vol.39 (1), p.26-34</ispartof><rights>Biomedical Engineering Society 2010</rights><rights>Biomedical Engineering Society 2011</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c588t-743bc667006535c8668ae40d72630c2487d3f34bd7ec40e670e4085d80dc97c33</citedby><cites>FETCH-LOGICAL-c588t-743bc667006535c8668ae40d72630c2487d3f34bd7ec40e670e4085d80dc97c33</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27923,27924</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20614244$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ikeda, Hideyoshi</creatorcontrib><creatorcontrib>Sumita, Yoshinori</creatorcontrib><creatorcontrib>Ikeda, Mihoko</creatorcontrib><creatorcontrib>Ikeda, Hisazumi</creatorcontrib><creatorcontrib>Okumura, Teruhito</creatorcontrib><creatorcontrib>Sakai, Eiko</creatorcontrib><creatorcontrib>Nishimura, Masahiro</creatorcontrib><creatorcontrib>Asahina, Izumi</creatorcontrib><title>Engineering Bone Formation from Human Dental Pulp- and Periodontal Ligament-Derived Cells</title><title>Annals of biomedical engineering</title><addtitle>Ann Biomed Eng</addtitle><addtitle>Ann Biomed Eng</addtitle><description>A robust method for inducing bone formation from cultured dental mesenchymal cells has not been established. In this study, a method for generating bone tissue
in vivo
from cultured human dental pulp- and periodontal ligament-derived cells (DPCs and PDLCs, respectively) was designed using exogenous bone morphogenetic protein 2 (BMP2). DPCs and PDLCs showed enhanced alkaline phosphatase (ALP) activity and calcified nodule formation in medium containing dexamethasone, β-glycerophosphate, and ascorbic acid (osteogenic medium). However, the addition of recombinant human bone morphogenetic protein 2 (rhBMP2) to osteogenic medium remarkably increased ALP activity and
in vitro
calcification above the increases observed with osteogenic medium alone. rhBMP2 also significantly upregulated the expression of
osteocalcin
,
osteopontin
, and
dentin matrix protein 1
mRNA in both cell types cultured in osteogenic medium. Finally, we detected prominent bone-like tissue formation
in vivo
when cells had been exposed to rhBMP2 in osteogenic medium. In contrast, treatments with osteogenic medium or rhBMP2 alone could not induce abundant mineralized tissue formation. We propose here that treatment with rhBMP2 in osteogenic medium can make dental mesenchymal tissues a highly useful source of cells for bone tissue engineering. In addition, both DPCs and PDLCs showed similar and remarkable osteo-inducibility.</description><subject>Biochemistry</subject><subject>Biocompatibility</subject><subject>Biological and Medical Physics</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedical Engineering and Bioengineering</subject><subject>Biomedical materials</subject><subject>Biomedicine</subject><subject>Biophysics</subject><subject>Bone Development - drug effects</subject><subject>Bone Development - physiology</subject><subject>Bone Morphogenetic Protein 2 - administration & dosage</subject><subject>Bones</subject><subject>Cell Differentiation</subject><subject>Cells, Cultured</subject><subject>Classical Mechanics</subject><subject>Coculture Techniques</subject><subject>Dental Pulp - cytology</subject><subject>Dental Pulp - drug effects</subject><subject>Dental Pulp - physiology</subject><subject>Human</subject><subject>Humans</subject><subject>In vivo testing</subject><subject>In vivo 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Eng</addtitle><date>2011-01-01</date><risdate>2011</risdate><volume>39</volume><issue>1</issue><spage>26</spage><epage>34</epage><pages>26-34</pages><issn>0090-6964</issn><eissn>1573-9686</eissn><abstract>A robust method for inducing bone formation from cultured dental mesenchymal cells has not been established. In this study, a method for generating bone tissue
in vivo
from cultured human dental pulp- and periodontal ligament-derived cells (DPCs and PDLCs, respectively) was designed using exogenous bone morphogenetic protein 2 (BMP2). DPCs and PDLCs showed enhanced alkaline phosphatase (ALP) activity and calcified nodule formation in medium containing dexamethasone, β-glycerophosphate, and ascorbic acid (osteogenic medium). However, the addition of recombinant human bone morphogenetic protein 2 (rhBMP2) to osteogenic medium remarkably increased ALP activity and
in vitro
calcification above the increases observed with osteogenic medium alone. rhBMP2 also significantly upregulated the expression of
osteocalcin
,
osteopontin
, and
dentin matrix protein 1
mRNA in both cell types cultured in osteogenic medium. Finally, we detected prominent bone-like tissue formation
in vivo
when cells had been exposed to rhBMP2 in osteogenic medium. In contrast, treatments with osteogenic medium or rhBMP2 alone could not induce abundant mineralized tissue formation. We propose here that treatment with rhBMP2 in osteogenic medium can make dental mesenchymal tissues a highly useful source of cells for bone tissue engineering. In addition, both DPCs and PDLCs showed similar and remarkable osteo-inducibility.</abstract><cop>Boston</cop><pub>Springer US</pub><pmid>20614244</pmid><doi>10.1007/s10439-010-0115-2</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Annals of biomedical engineering, 2011-01, Vol.39 (1), p.26-34 |
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| source | Springer Nature |
| subjects | Biochemistry Biocompatibility Biological and Medical Physics Biomedical and Life Sciences Biomedical Engineering and Bioengineering Biomedical materials Biomedicine Biophysics Bone Development - drug effects Bone Development - physiology Bone Morphogenetic Protein 2 - administration & dosage Bones Cell Differentiation Cells, Cultured Classical Mechanics Coculture Techniques Dental Pulp - cytology Dental Pulp - drug effects Dental Pulp - physiology Human Humans In vivo testing In vivo tests Osteogenesis - genetics Osteogenesis - physiology Periodontal Ligament - cytology Periodontal Ligament - drug effects Periodontal Ligament - physiology Proteins Surgical implants Tissue Engineering - methods |
| title | Engineering Bone Formation from Human Dental Pulp- and Periodontal Ligament-Derived Cells |
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