Gold–silver-alloy nanoprobes for one-pot multiplex DNA detection

A specific colorimetric DNA detection method based on oligonucleotide functionalized gold-silver-alloy nanoparticles (AuAg-alloy-nanoprobes) is presented. The AuAg-alloy-nanoprobes were then used for the specific detection of a DNA sequence from TP53-a gene involved in cancer development. The AuAg-a...

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Bibliographic Details
Published in:Nanotechnology 2010-06, Vol.21 (25), p.255101-255101
Main Authors: Doria, G, Larguinho, M, Dias, J T, Pereira, E, Franco, R, Baptista, P V
Format: Article
Language:English
Subjects:
Colorimetry
Colorimetry - methods
Deoxyribonucleic acid
DNA Probes - chemistry
DNA Probes - metabolism
DNA, Single-Stranded - analysis
DNA, Single-Stranded - metabolism
Gold Alloys - chemistry
Metal Nanoparticles - chemistry
Multiplexing
Nanocomposites
Nanomaterials
Nanoparticles
Nanostructure
Nucleic Acid Hybridization - methods
Oligonucleotides
Silver - chemistry
Strategy
Tumor Suppressor Protein p53 - genetics
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Summary:A specific colorimetric DNA detection method based on oligonucleotide functionalized gold-silver-alloy nanoparticles (AuAg-alloy-nanoprobes) is presented. The AuAg-alloy-nanoprobes were then used for the specific detection of a DNA sequence from TP53-a gene involved in cancer development. The AuAg-alloy-nanoprobes were then used in combination with Au-nanoprobes for a one-pot dual-colour detection strategy that allowed for the simultaneous differential detection of two distinct target sequences. This system poses an unprecedented opportunity to explore the combined use of metal nanoparticles with different composition towards the development of a multiplex one-pot colorimetric assay for DNA detection.
ISSN:0957-4484
1361-6528
DOI:10.1088/0957-4484/21/25/255101