Enzyme-assisted three phase partitioning: A novel approach for extraction of turmeric oleoresin

Three phase partitioning, a technique which has been explored for protein separation was evaluated for extraction of turmeric oleoresin. The process consists of simultaneous addition of t-butanol and ammonium sulphate to the aqueous slurry of turmeric powder. The extraction of oleoresin was optimize...

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Bibliographic Details
Published in:Process biochemistry (1991) 2011, Vol.46 (1), p.423-426
Main Authors: Kurmudle, Nilesh N., Bankar, Sandip B., Bajaj, Ishwar B., Bule, Mahesh V., Singhal, Rekha S.
Format: Article
Language:English
Subjects:
Acetone
alpha-amylase
Ammonium sulfate
Biochemistry
Enzyme assisted three phase partitioning
Extraction
glucan 1,4-alpha-glucosidase
Glucoamylase
Oleoresins
Partitioning
Slurries
Three phase
turmeric
Turmeric oleoresin
α-Amylase
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Summary:Three phase partitioning, a technique which has been explored for protein separation was evaluated for extraction of turmeric oleoresin. The process consists of simultaneous addition of t-butanol and ammonium sulphate to the aqueous slurry of turmeric powder. The extraction of oleoresin was optimized with respect to the concentration of ammonium sulphate loading and the ratio of t-butanol to slurry. Pretreatment of the slurries with a commercial enzyme preparation of α-amylase and/or glucoamylase followed by three phase partitioning was carried out. The extraction time with this technique is lower as compared to conventional acetone extraction.
ISSN:1359-5113
1873-3298
DOI:10.1016/j.procbio.2010.09.010