Hollow fiber-based liquid phase microextraction (HF-LPME) for a highly sensitive HPLC determination of sulfonamides and their main metabolites

• Published in: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Analytical technologies in the biomedical and life sciences, 2011-01, Vol.879 (2), p.197-204
• Main Authors: Ramos Payán, María, López, Miguel Ángel Bello, Fernández-Torres, Rut, Navarro, Mercedes Villar, Mochón, Manuel Callejón
• Format: Article
• Language: English
• Subjects: Analysis; Analytical, structural and metabolic biochemistry; Aqueous solutions; Arrays; Biological and medical sciences; Chemical Fractionation - methods; Chromatography, High Pressure Liquid - methods; Derivatives; drinking water; drug residues; Environmental water; fluorescence; Fundamental and applied biological sciences. Psychology; General pharmacology; Hafnium; HF-LPME; high performance liquid chromatography; Hollow fiber liquid phase microextraction; HPLC; Least-Squares Analysis; Liquid phases; liquid-phase microextraction; Medical sciences; Metabolites; Pharmacology. Drug treatments; Polypropylenes; Reproducibility of Results; Rivers - chemistry; sodium sulfate; sulfadiazine; sulfamerazine; sulfamethoxazole; Sulfonamides; Sulfonamides - analysis; Sulfonamides - chemistry; surface water; Waste water; wastewater; Water Pollutants, Chemical - analysis; Water Pollutants, Chemical - chemistry
• ISSN: 1570-0232; 1873-376X
• DOI: 10.1016/j.jchromb.2010.12.006

In this paper, three phase-hollow fiber-based liquid phase microextraction (HF-LPME) combined with a HPLC procedure using diode array (DAD) and fluorescence detection (FLD) has been developed for the determination of four widely used sulfonamides: sulfadiazine, sulfamerazine, sulfamethazine, sulfamethoxazole and their main metabolites, the corresponding N4-acetyl derivatives: N4-acetyl-sulfadiazine, N4-acetyl-sulfamerazine, N4-acetyl-sulfamethazine, N4-acetyl-sulfamethoxazole. A Q3/2 Accurel KM polypropylene hollow fiber supporting 1-octanol was used between a 2M Na2SO4 aqueous solution (pH 4) as a donor phase and aqueous solution (pH 12) as an acceptor phase. The procedure allows very low detection and quantitation limits of 0.3–33ngL−1 and 0.9–100ngL−1, respectively. The proposed method was applied to the determination of the analytes in environmental water samples (surface, tap and wastewater).