Characterization of Two Compatible Small Plasmids from Sphingobium yanoikuyae

We isolated and characterized two small cryptic indigenous plasmids, pYAN-1 (4,896 bp) and pYAN-2 (4,687 bp), from Sphingobium yanoikuyae, and developed a versatile system that permitted genetic manipulation of the genus Sphingomonas. Nucleotide sequencing of both plasmids revealed that they contain...

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Bibliographic Details
Published in:Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 2008-04, Vol.72 (4), p.1130-1133
Main Authors: OCHOU, Makoto, SAITO, Miyuki, KURUSU, Yasurou
Format: Article
Language:English
Subjects:
Antibiotics
Biochemistry
Biological and medical sciences
Biotechnology
Electroporation
electrporation
Fundamental and applied biological sciences. Psychology
Genes
Genetics
Novosphingobium
Nucleotides
Open Reading Frames
plasmid
Plasmids - genetics
Replication
Sequence Analysis, DNA
Sequence Homology, Amino Acid
Sphingobium
Sphingomonadaceae - genetics
Sphingomonas
Transformations
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Summary:We isolated and characterized two small cryptic indigenous plasmids, pYAN-1 (4,896 bp) and pYAN-2 (4,687 bp), from Sphingobium yanoikuyae, and developed a versatile system that permitted genetic manipulation of the genus Sphingomonas. Nucleotide sequencing of both plasmids revealed that they contained mobA, mobs, and repA genes, which are predicted to encode proteins associated with mobilization and replication, in common. Transformation with each plasmid harboring the antibiotic resistance gene by electroporation was fully successful, using Novosphingobium capsulatum as a host.
ISSN:0916-8451
1347-6947
1347-6947
DOI:10.1271/bbb.70813