Characterization of Two Compatible Small Plasmids from Sphingobium yanoikuyae

We isolated and characterized two small cryptic indigenous plasmids, pYAN-1 (4,896 bp) and pYAN-2 (4,687 bp), from Sphingobium yanoikuyae, and developed a versatile system that permitted genetic manipulation of the genus Sphingomonas. Nucleotide sequencing of both plasmids revealed that they contain...

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Published in:Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 2008-04, Vol.72 (4), p.1130-1133
Main Authors: OCHOU, Makoto, SAITO, Miyuki, KURUSU, Yasurou
Format: Article
Language:English
Subjects:
Antibiotics
Biochemistry
Biological and medical sciences
Biotechnology
Electroporation
electrporation
Fundamental and applied biological sciences. Psychology
Genes
Genetics
Novosphingobium
Nucleotides
Open Reading Frames
plasmid
Plasmids - genetics
Replication
Sequence Analysis, DNA
Sequence Homology, Amino Acid
Sphingobium
Sphingomonadaceae - genetics
Sphingomonas
Transformations
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cited_by cdi_FETCH-LOGICAL-c577t-57415cfd6503c6a2d92acec75d2ac9872bb567571592c1b91449eff577630b333
cites cdi_FETCH-LOGICAL-c577t-57415cfd6503c6a2d92acec75d2ac9872bb567571592c1b91449eff577630b333
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container_title Bioscience, biotechnology, and biochemistry
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creator OCHOU, Makoto
SAITO, Miyuki
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description We isolated and characterized two small cryptic indigenous plasmids, pYAN-1 (4,896 bp) and pYAN-2 (4,687 bp), from Sphingobium yanoikuyae, and developed a versatile system that permitted genetic manipulation of the genus Sphingomonas. Nucleotide sequencing of both plasmids revealed that they contained mobA, mobs, and repA genes, which are predicted to encode proteins associated with mobilization and replication, in common. Transformation with each plasmid harboring the antibiotic resistance gene by electroporation was fully successful, using Novosphingobium capsulatum as a host.
doi_str_mv 10.1271/bbb.70813
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source Free E-Journal (出版社公開部分のみ）; Oxford Journals Online
subjects Antibiotics
Biochemistry
Biological and medical sciences
Biotechnology
Electroporation
electrporation
Fundamental and applied biological sciences. Psychology
Genes
Genetics
Novosphingobium
Nucleotides
Open Reading Frames
plasmid
Plasmids - genetics
Replication
Sequence Analysis, DNA
Sequence Homology, Amino Acid
Sphingobium
Sphingomonadaceae - genetics
Sphingomonas
Transformations
title Characterization of Two Compatible Small Plasmids from Sphingobium yanoikuyae
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