Raman spectroscopy—A new method for the intra-operative assessment of axillary lymph nodes

Sentinel Lymph Node Biopsy has become the standard surgical procedure for the sampling of axillary lymph nodes in breast cancer. Intra operative node assessment is currently not offered to the majority of patients but would allow definitive axillary surgery to take place immediately. This would conf...

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Bibliographic Details
Published in:Analyst (London) 2010-01, Vol.135 (12), p.3042-3047
Main Authors: HORSNELL, Jonathan, STONELAKE, Paul, CHRISTIE-BROWN, Jonathan, SHETTY, Geeta, HUTCHINGS, Joanne, KENDALL, Catherine, STONE, Nicholas
Format: Article
Language:English
Subjects:
Analytical chemistry
Assessments
Axilla - pathology
Axilla - surgery
Breast
Breast Neoplasms - diagnosis
Breast Neoplasms - pathology
Breast Neoplasms - surgery
Cancer
Chemistry
Discriminant analysis
Exact sciences and technology
Female
Humans
Intraoperative Period
Lymph
Lymph Node Excision
Lymph Nodes - pathology
Lymph Nodes - surgery
Lymphatic Metastasis - pathology
Patients
Raman spectroscopy
Sentinel Lymph Node Biopsy - methods
Spectrometric and optical methods
Spectrum Analysis, Raman - methods
Surgery
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Summary:Sentinel Lymph Node Biopsy has become the standard surgical procedure for the sampling of axillary lymph nodes in breast cancer. Intra operative node assessment is currently not offered to the majority of patients but would allow definitive axillary surgery to take place immediately. This would confer benefits both to the patient and to the healthcare system. Our experimental study aims to demonstrate that a Raman spectroscopy probe device could overcome many of the disadvantages of current intra-operative analysis techniques. 38 axillary lymph nodes, 25 negative and 13 positive from 20 patients undergoing breast surgery for invasive breast cancer were assessed using a commercially available Raman spectroscopy probe. Spectra were assessed using principal component fed linear discriminant analysis trained by the histopathology results. Leave one node out cross validation achieved a sensitivity of up to 92% and a specificity of up to 100% in differentiating between normal and metastatic lymph nodes.
ISSN:0003-2654
1364-5528
DOI:10.1039/c0an00527d