Effects of Silencing Transforming Growth Factor-β1 by RNA Interference Plasmid on Rat Renal Allograft Fibrosis Using Smads Pathway

Objectives To evaluate the effects of transforming growth factor (TGF)-β1 RNA interference plasmid on rat renal allograft fibrosis and to explore its mechanisms. Methods A Sprague-Dawley to Wistar rat transplant kidney-sclerosis accelerated model was constructed and transfected with short hairpin RN...

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Bibliographic Details
Published in:Urology (Ridgewood, N.J.) N.J.), 2011-03, Vol.77 (3), p.762.e1-762.e7
Main Authors: Yin, Zhikang, Xia, Yuguo, Luo, Chunli, Zhang, Jiamo, He, Yunfeng, Wu, Xiaohou
Format: Article
Language:English
Subjects:
Animals
Blood Urea Nitrogen
Blotting, Western
Cadherins - metabolism
Collagen Type I - metabolism
Creatinine - blood
Down-Regulation - drug effects
Fibrosis
Immunohistochemistry
Kidney - metabolism
Kidney - pathology
Kidney Transplantation - pathology
Plasmids
Rats
Rats, Inbred WF
Rats, Sprague-Dawley
Reverse Transcriptase Polymerase Chain Reaction
RNA Interference
RNA, Small Interfering - pharmacology
Signal Transduction - drug effects
Smad3 Protein - metabolism
Smad7 Protein - metabolism
Transfection
Transforming Growth Factor beta1 - genetics
Transforming Growth Factor beta1 - metabolism
Transforming Growth Factor beta1 - pharmacology
Transplantation, Homologous
Up-Regulation - drug effects
Urology
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Summary:Objectives To evaluate the effects of transforming growth factor (TGF)-β1 RNA interference plasmid on rat renal allograft fibrosis and to explore its mechanisms. Methods A Sprague-Dawley to Wistar rat transplant kidney-sclerosis accelerated model was constructed and transfected with short hairpin RNA-TGF-β1 based on the hydromechanics. Kidney and blood samples were collected at the first, second, and third months after transplantation. Reverse transcriptase-polymerase chain reaction and Western blotting were used to detect the expression of TGF-β1, phosphorylated Smad3/7, E-cadherin, and type I collagen. The fibrosis extent was assessed using Masson staining. The immunohistochemical staining of E-cadherin and α-smooth muscle actin were used to label the tubular epithelial cells and fibroblast, respectively. Results The blood urea nitrogen and serum creatinine were lower in the plasmid group than in the control groups ( P
ISSN:0090-4295
1527-9995
DOI:10.1016/j.urology.2010.09.052