Native Fluorescence Spectroscopic Evaluation of Chemotherapeutic Effects on Malignant Cells using Nonnegative Matrix Factorization Analysis

The native fluorescence spectra of retinoic acid (RA)-treated and untreated human breast cancerous cells excited with the selective wavelengths of 300 nm and 340 nm were measured and analyzed using a blind source separation method namely Nonnegative Matrix Factorization (NMF). The results show that...

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Bibliographic Details
Published in:Technology in cancer research & treatment 2011-04, Vol.10 (2), p.113-120
Main Authors: Pu, Y., Tang, G. C., Wang, W. B., Savage, H. E., Schantz, S. P., Alfano, R. R.
Format: Article
Language:English
Subjects:
Algorithms
Antineoplastic Agents - pharmacology
Breast Neoplasms - chemistry
Breast Neoplasms - diagnosis
Cell Line, Tumor
Female
Flavins - chemistry
Humans
NAD - chemistry
Reference Standards
Sensitivity and Specificity
Spectrometry, Fluorescence - methods
Spectrometry, Fluorescence - standards
Tretinoin - pharmacology
Tryptophan - chemistry
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Summary:The native fluorescence spectra of retinoic acid (RA)-treated and untreated human breast cancerous cells excited with the selective wavelengths of 300 nm and 340 nm were measured and analyzed using a blind source separation method namely Nonnegative Matrix Factorization (NMF). The results show that the fluorophores of human malignant breast cells change their compositions when they are treated with RA. The reduced contribution from tryptophan, NADH and flavin to the fluorescence of the treated breast cancerous cells was observed in comparison with that of the untreated cells. The results indicate that the decrease of adenosine triphosphate (ATP) in the RA-treated cells. The possible clinical applications of this native fluorescence study are discussed.
ISSN:1533-0346
1533-0338
DOI:10.7785/tcrt.2012.500186