A new, very sensitive method of assessment of ptaquiloside, the major bracken carcinogen in the milk of farm animals

We describe a new method to detect trace levels of ptaquiloside (Pta), a major carcinogen of bracken fern in biological samples such as milk from farm animals. The method involves the absorption of analyte on carbograph followed by elution with solvents mixtures. The unstable analyte is then convert...

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Bibliographic Details
Published in:Food chemistry 2011-01, Vol.124 (2), p.660-665
Main Authors: Francesco, Bonadies, Giorgio, Berardi, Rosario, Nicoletti, Saverio, Romolo Francesco, Francesco, De Giovanni, Romano, Marabelli, Adriano, Santoro, Cinzia, Raso, Antonio, Tagarelli, Franco, Roperto, Valeria, Russo, Sante, Roperto
Format: Article
Language:English
Subjects:
animal health
asses
Bracken fern
Bromo-pterosine
carbograph
Carcinogens
cattle
dairy animals
detection
food contamination
gas chromatography
goats
mares
mass spectrometry
Milk
new methods
Ptaquiloside
ptaquilosides
Pteridium aquilinum
Pteridium spp
quantitative analysis
sheep
single ion monitoring mode
test sensitivity
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Summary:We describe a new method to detect trace levels of ptaquiloside (Pta), a major carcinogen of bracken fern in biological samples such as milk from farm animals. The method involves the absorption of analyte on carbograph followed by elution with solvents mixtures. The unstable analyte is then converted into Br-Pt (II), which is specific for Pta, as it is not a natural decay product of the glycoside in aqueous media. An internal standard, the Br-pterosine-d 2, prepared in our laboratories has been used. Detection and quantification are possible with gas chromatography/mass spectrometry (GC/MS) in single ion monitoring mode (SIM). The detectable amount is in the range of ppb. The method allowed us to detect Pta not only in the milk from bracken fern-poisoned cattle but also, for the first time, in the milk from healthy farm animals such as sheep, goat, horse, and donkey mares.
ISSN:0308-8146
1873-7072
DOI:10.1016/j.foodchem.2010.05.059