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complete genome sequence of Canna yellow streak virus

Canna yellow streak virus (Potyvirus, Potyviridae) was sequenced using the novel method of next-generation pyrosequencing. The complete genome was found to be 9,502 nucleotides excluding the poly-A tail with a predicted genome organisation typical for a member of the genus Potyvirus. As with other p...

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Bibliographic Details
Published in:Archives of virology 2010-09, Vol.155 (9), p.1515-1518
Main Authors: Monger, W. A, Adams, I. P, Glover, R. H, Barrett, B
Format: Article
Language:English
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Summary:Canna yellow streak virus (Potyvirus, Potyviridae) was sequenced using the novel method of next-generation pyrosequencing. The complete genome was found to be 9,502 nucleotides excluding the poly-A tail with a predicted genome organisation typical for a member of the genus Potyvirus. As with other potyviruses that infect monocotyledons, some of the predicted cleavage sites of the polyprotein genome were unusual, such as a glutamic acid/threonine (E/T) between the CI and 6K2 proteins and a glutamic acid/aspartic acid (E/D) between the NIa and NIb proteins. Evidence of the presence of endogenous pararetroviruses in the canna genome was found from the large number of sequences obtained with this method.
ISSN:0304-8608
1432-8798
DOI:10.1007/s00705-010-0694-0