Neutrophil activation during transmigration in vivo and in vitro

Neutrophil transmigration can be studied in vitro by use of the transwell model and in vivo by the skin chamber model. Activation during transmigration involves translocation of secretory vesicles and granules to the plasma- and phagolysosome membranes. In this study, we compared the skin chamber mo...

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Bibliographic Details
Published in:Journal of immunological methods 2010-09, Vol.361 (1-2), p.82-88
Main Authors: Paulsson, Josefin M., Jacobson, Stefan H., Lundahl, Joachim
Format: Article
Language:English
Subjects:
Degranulation
Neutrophil
Skin chamber
Transwell
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Summary:Neutrophil transmigration can be studied in vitro by use of the transwell model and in vivo by the skin chamber model. Activation during transmigration involves translocation of secretory vesicles and granules to the plasma- and phagolysosome membranes. In this study, we compared the skin chamber model with the transwell model, focusing on the mobilization of CR1 (CD35), CR3 (CD11b/CD18) and CD63 from intracellular vesicles and granules. In addition, functional responses towards a bacterial related stimulus, formyl-methionyl-leucyl-phenylalanine (fMLP), in terms of CR3 expression and production of reactive oxygen species (ROS) were assessed. Discrepancies between the skin chamber model and the transwell model were observed. The expression of CR1 increased following in vivo transmigration (p
ISSN:0022-1759
1872-7905
DOI:10.1016/j.jim.2010.07.015