Expression and secretion of Bluetongue virus serotype 8 (BTV-8)VP2 outer capsid protein by mammalian cells

VP2 is the outermost Bluetongue virus (BTV) antigenic protein, forming triskelion motifs on the virion surface. Although VP2 has been expressed successfully through many systems, its paracrine expression as a soluble form by mammalian cells represents a difficult task. In the present paper two fragm...

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Bibliographic Details
Published in:Journal of virological methods 2010-11, Vol.169 (2), p.420-424
Main Authors: Capocefalo, A., Franceschi, V., Mertens, P.P., Castillo-Olivares, J., Cavirani, S., Di Lonardo, E., Leni, Z., Donofrio, G.
Format: Article
Language:English
Subjects:
Biological and medical sciences
Bluetongue
Bluetongue virus
Bluetongue virus - genetics
Capsid Proteins - genetics
Capsid Proteins - secretion
Cell Line
Culture Media - chemistry
Expression
Fundamental and applied biological sciences. Psychology
Humans
Microbiology
Recombinant Fusion Proteins - genetics
Recombinant Fusion Proteins - secretion
Secretion
Techniques used in virology
Viral protein 2
Virology
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Summary:VP2 is the outermost Bluetongue virus (BTV) antigenic protein, forming triskelion motifs on the virion surface. Although VP2 has been expressed successfully through many systems, its paracrine expression as a soluble form by mammalian cells represents a difficult task. In the present paper two fragments of VP2 have been expressed successfully into the medium of transiently transfected mammalian cells through a fusion peptides strategy. The crude conditioned medium containing the secreted peptide could be employed for immunodiagnostic assay development or vaccine purposes.
ISSN:0166-0934
1879-0984
DOI:10.1016/j.jviromet.2010.08.002