Cardiovascular responses to l -glutamate microinjection into the hypothalamic paraventricular nucleus are mediated by a local nitric oxide-guanylate cyclase mechanism

• Published in: Brain research 2010-07, Vol.1344, p.87-95
• Main Authors: Busnardo, Cristiane, Crestani, Carlos C, Tavares, Rodrigo F, Resstel, Leonardo B.M, Correa, Fernando M.A
• Format: Article
• Language: English
• Subjects: Animals; Benzoates - pharmacology; Biological and medical sciences; Blood Pressure - drug effects; Blood pressure and heart rate; Carboxy-PTIO; Cardiovascular System - drug effects; Drug Interactions; Fundamental and applied biological sciences. Psychology; Glutamic Acid - pharmacology; Guanylate Cyclase - metabolism; Heart Rate - drug effects; Hypothalamus; Imidazoles - pharmacology; Male; Microinjections - methods; Motor control and motor pathways. Reflexes. Control centers of vegetative functions. Vestibular system and equilibration; N ω-Propyl- l-arginine; Neurology; Nitric oxide; Nitric Oxide - metabolism; Nitric Oxide Donors - pharmacology; Nitroprusside - pharmacology; ODQ; Oxadiazoles - pharmacology; Paraventricular Hypothalamic Nucleus - drug effects; Paraventricular Hypothalamic Nucleus - physiology; PVN; Quinoxalines - pharmacology; Rats; Rats, Wistar; Time Factors; Vertebrates: nervous system and sense organs; Wakefulness - drug effects
• ISSN: 0006-8993; 1872-6240
• DOI: 10.1016/j.brainres.2010.05.023

Abstract It has been reported that l -glutamate ( l -glu) microinjection into the hypothalamic paraventricular nucleus (PVN) evokes pressor and tachycardiac responses in unanesthetized rats. In the present study the hypothesis was tested that a local nitric oxide (NO)–guanylate cyclase interaction mediates cardiovascular effects of l -glu microinjection into the PVN of rats. The cardiovascular responses evoked by 10 nmol/100 nL of l -glu microinjected into the PVN were measured before and 10 min after PVN treatment with vehicle, the selective neuronal NO-synthase (nNOS) inhibitor Nω -Propyl- l -arginine (N-Propyl, 0.04 nmol or 4 nmol/100 nL), the NO scavenger carboxy-PTIO (C-PTIO, 1 nmol/100 nL) or the guanylate cyclase inhibitor 1H-[1,2,4] oxadiazolol [4,3- a ]quinoxalin-1-one (ODQ, 1 nmol/100 nL). In a final experiment, different doses of the NO donor sodium nitroprusside (SNP; 9, 27 or 45 nmol/100 nL) were microinjected into the PVN. Cardiovascular responses evoked by l -glu microinjection into the PVN were abolished by local pretreatment with N-Propyl in both anesthetized and unanesthetized rats. PVN treatment with either C-PTIO or ODQ also reduced l -glu cardiovascular responses. The microinjection of SNP into the PVN caused pressor and tachycardiac responses in unanesthetized rats, whereas depressor and bradycardiac responses were observed in anesthetized rats. The present results suggest that cardiovascular responses evoked by l -glu microinjection into the PVN involve a local production of NO and activation of guanylate cyclase.