Measuring photosynthetic parameters at a distance: laser induced fluorescence transient (LIFT) method for remote measurements of photosynthesis in terrestrial vegetation

We have developed a laser induced fluorescence transient (LIFT) technique and instrumentation to remotely measure photosynthetic properties in terrestrial vegetation at a distance of up to 50 m. The LIFT method uses a 665 nm laser to project a collimated, 100 mm diameter excitation beam onto leaves...

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Bibliographic Details
Published in:Photosynthesis research 2005-06, Vol.84 (1-3), p.121-129
Main Authors: Kolber, Z, Klimov, D, Ananyev, G, Rascher, U, Berry, J, Osmond, B
Format: Article
Language:English
Subjects:
Arabidopsis
Arabidopsis thaliana
electron transport chain
Fluorescence
Kinetics
laser induced fluorescence transient
Lasers
Light
non-photochemical quenching
optimal quantum yield of photosystem II
Photochemistry
Photosynthesis
Photosynthesis - physiology
photosystem II
Plant Leaves - metabolism
Populus - metabolism
Populus deltoides
quantum efficiency of photosystem II
Quercus
Quercus - metabolism
vegetation
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Summary:We have developed a laser induced fluorescence transient (LIFT) technique and instrumentation to remotely measure photosynthetic properties in terrestrial vegetation at a distance of up to 50 m. The LIFT method uses a 665 nm laser to project a collimated, 100 mm diameter excitation beam onto leaves of the targeted plant. Fluorescence emission at 690 nm is collected by a 250 mm reflective telescope and processed in real time to calculate the efficiency of photosynthetic light utilization, quantum efficiency of PS II, and the kinetics of photosynthetic electron transport. Operating with peak excitation power of 125 W m-2, and duty cycle of 10-50%, the instrument conforms to laser safety regulations. The LIFT instrument is controlled via an Internet connection, allowing it to operate from remote locations or platforms. Here we describe the theoretical basis of the LIFT methodology, and demonstrate its applications in remote measurements of photosynthetic properties in the canopy of cottonwood and oak trees, and in the rosette of Arabidopsis mutants.
ISSN:0166-8595
1573-5079
DOI:10.1007/s11120-005-5092-1