Evaluation of inoculum of Candida guilliermondii grown in presence of glucose on xylose reductase and xylitol dehydrogenase activities and xylitol production during batch fermentation of sugarcane bagasse hydrolysate

• Published in: Applied biochemistry and biotechnology 2005, Vol.121 (1-3), p.427-438
• Main Authors: Silva, D.D.V. da, Felipe, M. das G. de A, Mancilha, I.M. de, Silva, S.S. da
• Format: Article
• Language: English
• Subjects: Aldehyde Reductase - analysis; Aldehyde Reductase - biosynthesis; Aldehyde Reductase - chemistry; Bagasse; biological production; Candida - growth & development; Candida - metabolism; Candida guilliermondii; Carbon sources; Cell Culture Techniques - methods; Cell Proliferation; Culture Media - analysis; Culture Media - chemistry; Culture Media - metabolism; D-Xylulose Reductase; Dehydrogenase; Enzymatic activity; Enzyme Activation; Enzymes; Fermentation; Glucose; Glucose - metabolism; Metabolic Clearance Rate; Metabolism; Studies; Sugar Alcohol Dehydrogenases - analysis; Sugar Alcohol Dehydrogenases - biosynthesis; Sugar Alcohol Dehydrogenases - chemistry; Sugarcane; Xylitol - metabolism; Xylose - metabolism; Yeast
• ISSN: 0273-2289; 1559-0291; 0273-2289
• DOI: 10.1385/ABAB:121:1-3:0427

The effect of glucose on xylose-xylitol metabolism in fermentation medium consisting of sugarcane bagasse hydrolysate was evaluated by employing an inoculum of Candida guilliermondii grown in synthetic media containing, as carbon sources, glucose (30 g/L), xylose (30 g/L), or a mixture of glucose (2 g/L) and xylose (30 g/L). The inoculum medium containing glucose promoted a 2.5-fold increase in xylose reductase activity (0.582 IU/mg(prot)) and a 2-fold increase in xylitol dehydrogenase activity (0.203 IU/mg(prot)) when compared with an inoculum-grown medium containing only xylose. The improvement in enzyme activities resulted in higher values of xylitol yield (0.56 g/g) and productivity (0.46 g/[L.h]) after 48 h of fermentation.