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Biodiesel Fuel Production by the Transesterification Reaction of Soybean Oil Using Immobilized Lipase

The enzymatic alcoholysis of soybean oil with methanol and ethanol was investigated using a commercial, immobilized lipase (Lipozyme RMIM). The effect of alcohol (methanol or ethanol), enzyme concentration, molar ratio of alcohol to soybean oil, solvent, and temperature on biodiesel production was d...

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Bibliographic Details
Published in:Applied biochemistry and biotechnology 2007-04, Vol.137-140 (1-12), p.105-114
Main Authors: BERNARDES, Otavio L, BEVLLAQUA, Juliana V, LEAL, Marcia C. M. R, FREIRE, Denise M. G, LANGONE, Marta A. P
Format: Article
Language:English
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Summary:The enzymatic alcoholysis of soybean oil with methanol and ethanol was investigated using a commercial, immobilized lipase (Lipozyme RMIM). The effect of alcohol (methanol or ethanol), enzyme concentration, molar ratio of alcohol to soybean oil, solvent, and temperature on biodiesel production was determined. The best conditions were obtained in a solvent-free system with ethanol/oil molar ratio of 3.0, temperature of 50 degrees C, and enzyme concentration of 7.0% (w/w). Three-step batch ethanolysis was most effective for the production of biodiesel. Ethyl esters yield was about 60% after 4 h of reaction.
ISSN:0273-2289
1559-0291
DOI:10.1007/s12010-007-9043-5