The Influence of the Environment on Cajal–Retzius Cell Migration

• Published in: Cerebral cortex (New York, N.Y. 1991) N.Y. 1991), 2010-10, Vol.20 (10), p.2348-2360
• Main Authors: Ceci, María Laura, López-Mascaraque, Laura, de Carlos, Juan A.
• Format: Article
• Language: English
• Subjects: Animals; Benzopyrans - metabolism; Brain Tissue Transplantation - methods; Cell Differentiation - physiology; Cell Movement - genetics; Cell Movement - physiology; Cerebral Cortex - cytology; Cerebral Cortex - embryology; Cerebral Cortex - transplantation; culture; development; embryo; Embryo, Mammalian; Eye Proteins - genetics; Female; Fluoresceins - metabolism; Gene Expression Regulation, Developmental - physiology; Green Fluorescent Proteins - genetics; Homeodomain Proteins - genetics; In Vitro Techniques; Mice; Mice, Inbred C57BL; Mice, Transgenic; mouse; Mutation - genetics; Nerve Tissue Proteins - genetics; Nerve Tissue Proteins - metabolism; Neurons - classification; Neurons - physiology; Paired Box Transcription Factors - genetics; Pax6; PAX6 Transcription Factor; Pregnancy; Repressor Proteins - genetics; tangential migration; tissue implants
• ISSN: 1047-3211; 1460-2199
• DOI: 10.1093/cercor/bhp305

During cerebral cortex development, different cell populations migrate tangentially through the preplate, traveling from their site of origin toward their final positions. One of the earliest populations formed, the Cajal–Retzius (C-R) cells, is mainly generated in different cortical hem (CH) domains, and they migrate along established and parallel routes to cover the whole cortical mantle. In this study, we present evidence that the phenotype of –Retzius cells, as well as some of their migratory characteristics, is specified in the area where the cells are generated. Nevertheless, when implanted ectopically, these cells can follow new migratory routes, indicating that locally provided genetic cues along the migratory path nonautonomously influence the position of these cells emanating from different portions of the CH. This was witnessed by performing CH implants of tissue expressing fluorescent tracers in live whole embryos. In the same way, tracer injections into the hem of Small eye mutant mice were particularly informative since the lack of Pax6 affects some guidance factors in the migratory environment. As a result, in these animals, the C-R cell population is disorganized, and it forms 1 day late, showing certain differences in gene expression that might help explain these disruptions.