Cytochrome-b variation in Apis mellifera samples and its association with COI-COII patterns

Five Mbo I (Mbo-A, Mbo-M, Mbo-C¹, Mbo-C² and Mbo-C³) and Hinf I (Hinf-1 to Hinf-5) patterns were observed in Apis mellifera samples after restriction of a 485 bp fragment of the mitochondrial cytochrome-b (cyt-b) gene. Associating the cyt-b Restriction fragment length polymorphism (RFLP) pattern of...

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Bibliographic Details
Published in:Genetica 2009-03, Vol.135 (2), p.149-155
Main Authors: Ferreira, Kátia M, e Silva, Otávio Lino, Arias, Maria C, Del Lama, Marco A
Format: Article
Language:English
Subjects:
Animal Genetics and Genomics
Animals
Apis mellifera
Bees - enzymology
Bees - genetics
Biomedical and Life Sciences
Composite haplotypes
Cytochrome
Cytochrome-b
Cytochromes b - genetics
DNA, Mitochondrial - genetics
Electron Transport Complex IV - genetics
Electron Transport Complex IV - metabolism
Evolutionary Biology
Genetic Variation
Haplotypes
Human Genetics
Life Sciences
Microbial Genetics and Genomics
mitochondrial DNA
Plant Genetics and Genomics
Polymorphism, Restriction Fragment Length
Restriction Mapping
RFLP-PCR
Sequence Analysis, DNA
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Summary:Five Mbo I (Mbo-A, Mbo-M, Mbo-C¹, Mbo-C² and Mbo-C³) and Hinf I (Hinf-1 to Hinf-5) patterns were observed in Apis mellifera samples after restriction of a 485 bp fragment of the mitochondrial cytochrome-b (cyt-b) gene. Associating the cyt-b Restriction fragment length polymorphism (RFLP) pattern of each sample to its respective previously established COI-COII (Dra I sites) pattern, five restriction patterns (Mbo-C¹, Mbo-C², Mbo-C³, Hinf-1 and Hinf-4) were observed in samples of maternal origin associated to the evolutionary branch C. No deletions or insertions were observed and the nucleotide substitution rate was estimated at 5.4%. Higher nucleotide diversity was observed among the branch C-haplotypes when compared with A and M lineages. Further studies are needed to confirm if the cyt-b + COI-COII haplotypes help to assign certain phylogeographic patterns to the branch C and to clarify phylogenetic relationships among A. mellifera subspecies.
ISSN:0016-6707
1573-6857
DOI:10.1007/s10709-008-9264-8