Large-scale functional analysis using peptide or protein arrays

The array format for analyzing peptide and protein function offers an attractive experimental alternative to traditional library screens. Powerful new approaches have recently been described, ranging from synthetic peptide arrays to whole proteins expressed in living cells. Comprehensive sets of pur...

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Bibliographic Details
Published in:Nature biotechnology 2000-04, Vol.18 (4), p.393-397
Main Authors: Cagney, Gerard, Emili, Alia Qureshi
Format: Article
Language:English
Subjects:
Agriculture
Arrays
Bioinformatics
Biological and medical sciences
Biomedical and Life Sciences
Biomedical Engineering/Biotechnology
Biomedicine
Biotechnology
Deoxyribonucleic acid
Diverse techniques
DNA
Fundamental and applied biological sciences. Psychology
Genetic engineering
Genetic screening
Genomes
high-throughput screening
Life Sciences
Molecular and cellular biology
Peptide Library
Peptides
Peptides - chemical synthesis
Peptides - chemistry
Peptides - metabolism
protein arrays
Proteins
Proteins - chemistry
Proteins - metabolism
review-article
Yeasts
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Summary:The array format for analyzing peptide and protein function offers an attractive experimental alternative to traditional library screens. Powerful new approaches have recently been described, ranging from synthetic peptide arrays to whole proteins expressed in living cells. Comprehensive sets of purified peptides and proteins permit high-throughput screening for discrete biochemical properties, whereas formats involving living cells facilitate large-scale genetic screening for novel biological activities. In the past year, three major genome-scale studies using yeast as a model organism have investigated different aspects of protein function, including biochemical activities, gene disruption phenotypes, and protein–protein interactions. Such studies show that protein arrays can be used to examine in parallel the functions of thousands of proteins previously known only by their DNA sequence.
ISSN:1087-0156
1546-1696
DOI:10.1038/74442