Sporamin induce apoptosis in human tongue carcinoma cells by down-regulating Akt/GSK-3 signaling

We investigated the effects of sporamin, the major soluble protein with a kunitz‐type trypsin inhibitory activity in the root tuber of the sweet potato, on cell proliferation, apoptosis, Akt/GSK‐3 signaling and its related genes to provide more insights in the mechanism behind the inhibitory effects...

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Bibliographic Details
Published in:Fundamental & clinical pharmacology 2011-04, Vol.25 (2), p.229-236
Main Authors: Yao, Jun, Qian, Cuijuan
Format: Article
Language:English
Subjects:
Akt
Antineoplastic Agents - administration & dosage
Antineoplastic Agents - pharmacology
apoptosis
Apoptosis - drug effects
Biological and medical sciences
Cell Line, Tumor
Cell Proliferation - drug effects
Dose-Response Relationship, Drug
Down-Regulation - drug effects
Glycogen Synthase Kinase 3 - drug effects
Glycogen Synthase Kinase 3 - metabolism
GSK-3
Humans
Medical sciences
Otorhinolaryngology. Stomatology
Pharmacology. Drug treatments
Phosphorylation - drug effects
Plant Proteins - administration & dosage
Plant Proteins - pharmacology
Proto-Oncogene Proteins c-akt - drug effects
Proto-Oncogene Proteins c-akt - metabolism
Signal Transduction - drug effects
sporamin
tongue cancer
Tongue Neoplasms - drug therapy
Tongue Neoplasms - pathology
Tumors
Upper respiratory tract, upper alimentary tract, paranasal sinuses, salivary glands: diseases, semeiology
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Summary:We investigated the effects of sporamin, the major soluble protein with a kunitz‐type trypsin inhibitory activity in the root tuber of the sweet potato, on cell proliferation, apoptosis, Akt/GSK‐3 signaling and its related genes to provide more insights in the mechanism behind the inhibitory effects of sporamin in a human tongue cancer line Tca8113. In this study, sporamin inhibited cell proliferation and induced apoptosis in Tca8113 cells in a concentration‐dependent and time‐dependent manner. Consistently, Bax was up‐regulated and Bcl‐2 was down‐regulated in sporamin‐treated cells. Furthermore, Akt/GSK‐3 signaling was down‐regulated in sporamin‐treated cells. Consistently, the phosphorylated Bad was significantly declined in sporamin‐treated Tca8113 cells. These results suggest the antiproliferative effects of sporamin in Tca8113 cells might result partly from induction of apoptosis by down‐regulating Akt/GSK‐3 pathway.
ISSN:0767-3981
1472-8206
DOI:10.1111/j.1472-8206.2010.00830.x