Study of DNA damage with a new system for irradiation of samples in a nuclear reactor

In this paper, we report results of a quantitative analysis of the effects of neutrons on DNA, and, specifically, the production of simple and double breaks of plasmid DNA in aqueous solutions with different concentrations of free-radical scavengers. The radiation damage to DNA was evaluated by elec...

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Bibliographic Details
Published in:Applied radiation and isotopes 2011-02, Vol.69 (2), p.373-376
Main Authors: Gual, Maritza R., Milian, Felix M., Deppman, Airton, Coelho, Paulo R.P.
Format: Article
Language:English
Subjects:
DNA
DNA - radiation effects
DNA Damage
Dose-Response Relationship, Radiation
Free Radical Scavengers - chemistry
Gamma Rays
Neutrons
Nuclear Reactors
Plasmid
Radiation damage
Reactor
Sample transportation system
Thermoluminescent Dosimetry
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Summary:In this paper, we report results of a quantitative analysis of the effects of neutrons on DNA, and, specifically, the production of simple and double breaks of plasmid DNA in aqueous solutions with different concentrations of free-radical scavengers. The radiation damage to DNA was evaluated by electrophoresis through agarose gels. The neutron and gamma doses were measured separately with thermoluminescent detectors. In this work, we have also demonstrated usefulness of a new system for positioning and removing samples in channel BH#3 of the IEA-R1 reactor at the Instituto de Pesquisas Energéticas e Nucleares (Brazil) without necessity of interrupting the reactor operation.
ISSN:0969-8043
1872-9800
DOI:10.1016/j.apradiso.2010.10.025