Prevalence and molecular diagnosis of Trypanosoma evansi in Nili-Ravi buffalo (Bubalus bubalis) in different districts of Punjab (Pakistan)

The prevalence of Trypanosoma evansi was investigated in 1,250 Nili-Ravi buffaloes of mixed age and sex by polymerase chain reaction (PCR) for the first time in Pakistan. DNA of the trypanosomes was isolated with TRIREAGENT®. The assay was employed using primers ESAG 6/7, specific for a 237-bp fragm...

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Bibliographic Details
Published in:Tropical animal health and production 2010-12, Vol.42 (8), p.1597-1599
Main Authors: Shahzad, Waseem, Munir, Rashid, Khan, Mohammad S, Ahmad, Mansur D, Ijaz, Mohammad, Ahmad, Ashfaq, Iqbal, Mohammad
Format: Article
Language:English
Subjects:
Animals
Biomedical and Life Sciences
Brief Communication
Bubalus bubalis
Buffaloes - parasitology
cattle
Deoxyribonucleic acid
DNA
DNA Primers - genetics
Female
Life Sciences
Male
Pakistan - epidemiology
polymerase chain reaction
Polymerase Chain Reaction - veterinary
Prevalence
Sensitivity and Specificity
T. evansi
Trypanosoma
Trypanosoma - genetics
Trypanosoma evansi
trypanosomiasis
Trypanosomiasis - epidemiology
Trypanosomiasis - veterinary
Veterinary Medicine/Veterinary Science
Zoology
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Summary:The prevalence of Trypanosoma evansi was investigated in 1,250 Nili-Ravi buffaloes of mixed age and sex by polymerase chain reaction (PCR) for the first time in Pakistan. DNA of the trypanosomes was isolated with TRIREAGENT®. The assay was employed using primers ESAG 6/7, specific for a 237-bp fragment from T. evansi genomic DNA. The samples were screened for the presence of T. evansi also by stained thin smear. Forty-four (3.5%) samples were positive by microscopy, while 97 (7.7%) samples were identified by PCR, indicating the high sensitivity of PCR for surveying the disease in epidemiological studies.
ISSN:0049-4747
1573-7438
DOI:10.1007/s11250-010-9616-0