Comparative study on responses of cattle and water buffalo (Bubalus bubalis) to experimental inoculation of Brucella abortus biovar 1 by the intraconjunctival route—a preliminary report

The preliminary study was conducted to assess the virulence of a strain of Brucella abortus (1969D) and to compare the susceptibility of water buffalo and cattle calves to infection by the intraconjunctival route. Seven of each cattle and water buffalo (Bubalus bubalis) calves aged 3-6 months were i...

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Bibliographic Details
Published in:Tropical animal health and production 2010-12, Vol.42 (8), p.1685-1694
Main Authors: Adesiyun, Abiodun A, Fosgate, Geoff T, Persad, Anil, Campbell, Mervyn, Seebaransingh, Ravi, Stewart-Johnson, Alva
Format: Article
Language:English
Subjects:
Animals
biological resistance
Biomedical and Life Sciences
Brucella abortus
Brucella abortus - pathogenicity
Brucella melitensis biovar Abortus
Brucellosis, Bovine - pathology
Bubalus bubalis
Buffaloes
Cattle
Colony Count, Microbial - veterinary
Comparative studies
Conjunctiva - microbiology
Enzyme-Linked Immunosorbent Assay - veterinary
Female
Intraconjunctival
Life Sciences
Lymph nodes
Lymph Nodes - microbiology
Lymph Nodes - pathology
Male
Original Research
Serologic Tests - veterinary
Species Specificity
Statistics, Nonparametric
Veterinary Medicine/Veterinary Science
Virulence
Zoology
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Summary:The preliminary study was conducted to assess the virulence of a strain of Brucella abortus (1969D) and to compare the susceptibility of water buffalo and cattle calves to infection by the intraconjunctival route. Seven of each cattle and water buffalo (Bubalus bubalis) calves aged 3-6 months were inoculated intraconjunctivally with counts ranging from 1.5 × 10⁷ to 1.7 × 10¹⁰ colony forming units of B. abortus. Animals were monitored over an 8-week period for clinical manifestations and serological and hematological evidence of infection. At slaughter, eight lymph nodes from each animal were sampled for bacteriological and histopathological assessments. Lymph nodes from three water buffalo (43%) and five cattle (71%) yielded B. abortus (P = 0.048). Parotid/prescapular lymph nodes were most sensitive in detecting B. abortus. Our data suggest that B. abortus strain 1969D may be used as challenge strain, and water buffalo appeared to have a lower susceptibility to B. abortus infection than cattle.
ISSN:0049-4747
1573-7438
DOI:10.1007/s11250-010-9621-3