Antibiotics promoting oxidative stress inhibit formation of Escherichia coli biofilm via indole signalling

Recent studies have revealed that antibiotics can promote the formation of reactive oxygen species which contribute to cell death. In this study, we report that five different antibiotics known to stimulate production of reactive oxygen species inhibited growth of Escherichia coli biofilm. We demons...

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Bibliographic Details
Published in:Research in microbiology 2010-12, Vol.161 (10), p.847-853
Main Authors: Kuczyńska-Wiśnik, Dorota, Matuszewska, Ewelina, Furmanek-Blaszk, Beata, Leszczyńska, Daria, Grudowska, Alicja, Szczepaniak, Paweł, Laskowska, Ewa
Format: Article
Language:English
Subjects:
Anti-Bacterial Agents - pharmacology
Antibiotics
Bacteriology
Biofilm
Biofilms
Biofilms - drug effects
Biofilms - growth & development
Biological and medical sciences
Cell death
Dimethyl sulfoxide
Dimethyl Sulfoxide - metabolism
Escherichia coli
Escherichia coli - drug effects
Escherichia coli - growth & development
Escherichia coli - physiology
Free Radical Scavengers - metabolism
Fundamental and applied biological sciences. Psychology
Gene Expression Profiling
Hydrogen peroxide
Indole
Indoles - metabolism
Microbiology
Miscellaneous
Oxidative Stress
Reactive oxygen species
Reactive Oxygen Species - metabolism
Signal Transduction
Tryptophan 2,3-dioxygenase
Tryptophanase - biosynthesis
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Summary:Recent studies have revealed that antibiotics can promote the formation of reactive oxygen species which contribute to cell death. In this study, we report that five different antibiotics known to stimulate production of reactive oxygen species inhibited growth of Escherichia coli biofilm. We demonstrated that supression of biofilm formation was mainly a consequence of the increase in the extracellular concentration of indole, a signal molecule which suppresses growth of bacterial biofilm. Indole production was enhanced under antibiotic-mediated oxidative stress due to overexpression of tryptophanase (TnaA), which catalyzes synthesis of indole. We found that DMSO (dimethyl sulfoxide), a hydrogen peroxide scavenger, or the lack of trypthophanase, which catalyzes production of indole, partly restored formation of E. coli biofilm in the presence of antibiotics. In conclusion, these findings confirmed that antibiotics which promote formation of ROS (reactive oxygen species) can inhibit development of E. coli biofilm in an indole-dependent process.
ISSN:0923-2508
1769-7123
DOI:10.1016/j.resmic.2010.09.012