Expression of Recombinant Potato leafroll virus Structural and Non-structural Proteins for Antibody Production

Vector pMPM-A4Ω and vectors pQE-30 and pET-45b(+) containing the 6x His-tag sequence were used for expression of Potato leafroll virus (PLRV) structural and non-structural proteins in Escherichia coli. Coat protein (CP) and RNA-dependent RNA polymerase (RdRp)-fragments RdRp₄₃-₆₁₆ and RdRp₃₀₄-₅₃₇ wer...

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Bibliographic Details
Published in:Journal of phytopathology 2011-02, Vol.159 (2), p.130-132
Main Authors: Plchova, Helena, Moravec, Tomas, Dedic, Petr, Cerovska, Noemi
Format: Article
Language:English
Subjects:
antibody production
Biological and medical sciences
Fundamental and applied biological sciences. Psychology
Indexing in process
Phytopathology. Animal pests. Plant and forest protection
Plant viruses and viroids
Potato leafroll virus
recombinant viral antigen
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Summary:Vector pMPM-A4Ω and vectors pQE-30 and pET-45b(+) containing the 6x His-tag sequence were used for expression of Potato leafroll virus (PLRV) structural and non-structural proteins in Escherichia coli. Coat protein (CP) and RNA-dependent RNA polymerase (RdRp)-fragments RdRp₄₃-₆₁₆ and RdRp₃₀₄-₅₃₇ were chosen for expression. A high level of CP and RdRp₃₀₄-₅₃₇ was obtained only in an expression system using pET-45b(+) vector and E. coli Rosetta-gami 2(DE3) cells. After purification, the His-tagged PLRV proteins were used for immunization of rabbits.
ISSN:0931-1785
1439-0434
DOI:10.1111/j.1439-0434.2010.01740.x