Mutation of a degS Homologue in Enterobacter cloacae Decreases Colonization and Biological Control of Damping-Off on Cucumber

• Published in: Phytopathology 2011-02, Vol.101 (2), p.271-280
• Main Authors: Roberts, Daniel P, Lohrke, Scott M, McKenna, Laurie, Lakshman, Dilip K, Kong, Hyesuk, Lydon, John
• Format: Article
• Language: English
• Subjects: Amino Acid Sequence; Bacterial Proteins - genetics; Bacterial Proteins - physiology; Biological and medical sciences; Cucumis sativus; Cucumis sativus - genetics; Cucumis sativus - microbiology; DNA, Bacterial; Enterobacter cloacae; Enterobacter cloacae - enzymology; Enterobacter cloacae - genetics; Enterobacter cloacae - growth & development; Escherichia coli; Escherichia coli - genetics; Fundamental and applied biological sciences. Psychology; Genes, Bacterial; Molecular Sequence Data; Mutagenesis, Insertional - physiology; Pest Control, Biological; Phytopathology. Animal pests. Plant and forest protection; Plant Diseases - microbiology; Plant Extracts - pharmacology; Plant Roots - microbiology; Pythium - growth & development; Pythium - pathogenicity; Pythium ultimum; Seeds - microbiology; Stress, Physiological
• ISSN: 0031-949X; 1943-7684
• DOI: 10.1094/PHYTO-03-10-0076

We have been using mutagenesis to determine how biocontrol bacteria such as Enterobacter cloacae 501R3 deal with complex nutritional environments found in association with plants. E. cloacae C10, a mutant of 501R3 with a transposon insertion in degS, was diminished in growth on synthetic cucumber root exudate (SRE), colonization of cucumber seed and roots, and control of damping-off of cucumber caused by Pythium ultimum. DegS, a periplasmic serine protease in the closely related bacterium Escherichia coli K12, is required for the RpoE-mediated stress response. C10 containing wild-type degS from 501R3 or from E. coli K12 on pBeloBAC11 was significantly increased in growth on SRE, colonization of cucumber roots, and control of P. ultimum relative to C10 containing pBeloBAC11 alone. C10 and 501R3 were similar in sensitivity to acidic conditions, plant-derived phenolic compounds, oxidative stress caused by hydrogen peroxide, dessication, and high osmoticum; stress conditions potentially associated with plants. This study demonstrates a role for degS in the spermosphere and rhizosphere during colonization and disease control by Enterobacter cloacae. This study implicates, for the first time, the involvement of DegS and, by extension, the RpoE-mediated stress response, in reducing stress on E. cloacae resulting from the complex nutritional environments in the spermosphere and rhizosphere.