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Methods for Conducting Bioassays Using Embryos and Larvae of Pacific Herring, Clupea pallasi

The rapid decrease of several stocks of Pacific herring, Clupea pallasi, in Puget Sound, Washington, has led to concerns about the effects of industrial and nonpoint source contamination on the embryo and larval stages of this and related forage fish species. To address these concerns, the state of...

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Published in:Archives of environmental contamination and toxicology 2011-02, Vol.60 (2), p.290-308
Main Authors: Dinnel, Paul A, Middaugh, Douglas P, Schwarck, Nathan T, Farren, Heather M, Haley, Richard K, Hoover, Richard A, Elphick, James, Tobiason, Karen, Marshall, Randall R
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creator Dinnel, Paul A
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description The rapid decrease of several stocks of Pacific herring, Clupea pallasi, in Puget Sound, Washington, has led to concerns about the effects of industrial and nonpoint source contamination on the embryo and larval stages of this and related forage fish species. To address these concerns, the state of Washington and several industries have funded efforts to develop embryo and larval bioassay protocols that can be used by commercial laboratories for routine effluent testing. This article presents the results of research to develop herring embryo and larval bioassay protocols. Factors evaluated during protocol development included temperature, salinity, dissolved oxygen (DO), light intensity, photoperiod, larval feeding regimes, use of brine and artificial sea salts, gonad sources, collection methods, and egg quality.
doi_str_mv 10.1007/s00244-010-9600-8
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subjects Animal, plant and microbial ecology
Animals
Applied ecology
Bioassay
Bioassays
Biological and medical sciences
Biological Assay - methods
Clupea
Contamination
Dissolution
Dissolved oxygen
Earth and Environmental Science
Ecology
Ecotoxicology
Ecotoxicology, biological effects of pollution
Eggs
Embryo, Nonmammalian - chemistry
Embryo, Nonmammalian - drug effects
Embryos
Environment
Environmental Chemistry
Environmental Health
Fishes - physiology
Fundamental and applied biological sciences. Psychology
General aspects
Gonads
Herrings
Indicator organisms
Industrial pollution
Laboratories
Larva - chemistry
Larva - drug effects
Larvae
Light intensity
Marine
Monitoring/Environmental Analysis
Nonpoint source pollution
Oil spills
PCB
Pollution
Polychlorinated biphenyls
Protocol
Raw materials
Salinity
Seawater - analysis
Seawater - chemistry
Sediments
Soil Science & Conservation
Studies
Test methods
Toxicity
Toxicity Tests, Acute - methods
Toxicity Tests, Chronic - methods
Washington
Water Pollutants, Chemical - analysis
Water Pollutants, Chemical - chemistry
Water Pollutants, Chemical - toxicity
Water Pollution - analysis
title Methods for Conducting Bioassays Using Embryos and Larvae of Pacific Herring, Clupea pallasi
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