Effects of gene augmentation on the removal of 2,4-dichlorophenoxyacetic acid in a biofilm reactor under different scales and substrate conditions

With a conjugative plasmid pJP4 carrying strain as the donor, two bioaugmentation experiments were conducted in a microcosm biofilm reactor with 2,4-D as the sole carbon source operated in fed-batch mode, and an enlarged lab-scale sequence batch biofilm reactor with mixed carbon sources of 2,4-D and...

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Bibliographic Details
Published in:Journal of hazardous materials 2011-01, Vol.185 (2), p.689-695
Main Authors: Quan, Xiangchun, Tang, Hua, Ma, Jingyun
Format: Article
Language:English
Subjects:
2,4-D
2,4-Dichlorophenoxyacetic acid
2,4-Dichlorophenoxyacetic Acid - isolation & purification
Applied sciences
Augmentation
Bioaugmentation
biodegradability
Biodegradation
biofilm
Biofilms
Bioreactors
biotransformation
Carbon
Chemical engineering
Degradation
Electrophoresis, Agar Gel
Exact sciences and technology
Expansion
Gene horizontal transfer
Genes
Genes, Bacterial
microbial communities
Microorganisms
Microscopy, Confocal
Plasmid pJP4
Plasmids
Pollution
Polymerase Chain Reaction
Pseudomonas putida - genetics
Pseudomonas putida - metabolism
Reactors
Sequence batch biofilm reactor (SBBR)
Sole
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Summary:With a conjugative plasmid pJP4 carrying strain as the donor, two bioaugmentation experiments were conducted in a microcosm biofilm reactor with 2,4-D as the sole carbon source operated in fed-batch mode, and an enlarged lab-scale sequence batch biofilm reactor with mixed carbon sources of 2,4-D and other easily biodegradable compounds, respectively. In the microcosm study under sole carbon source condition, bioaugmentation led to a persistently increased 2,4-D degradation rate in the five operation cycles with enhancement of 13–64%. For the enlarged lab-scale bioaugmentation experiment under mixed carbon source conditions, no enhancement in 2,4-D removal could be observed during start-up period. After a period of operation, biofilm samples from the bioaugmented reactor demonstrated a stronger degradation capacity than the control and showed the presence of a large number of transconjugants. This study indicates that bioaugmentation based on plasmid horizontal transfer is a feasible strategy to establish functional microbial community in a biofilm reactor, and the strong selective pressure of 2,4-D existing alone and persistently was more favorable for the success of gene augmentation.
ISSN:0304-3894
1873-3336
DOI:10.1016/j.jhazmat.2010.09.073