Microbial community analysis of perchlorate-reducing cultures growing on zero-valent iron

Anaerobic microbial mixed cultures demonstrated its ability to completely remove perchlorate in the presence of zero-valent iron. In order to understand the major microbial reaction in the iron-supported culture, community analysis comprising of microbial fatty acids and polymerase chain reaction–de...

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Bibliographic Details
Published in:Journal of hazardous materials 2011-01, Vol.185 (2), p.669-676
Main Authors: Son, Ahjeong, Schmidt, Carl J., Shin, Hyejin, Cha, Daniel K.
Format: Article
Language:English
Subjects:
Applied sciences
Bacteria
Bacteria, Anaerobic - growth & development
Bacteria, Anaerobic - metabolism
Base Sequence
Bioreactors
chemotaxonomy
Chlorite dismutase (cld) gene
Communities
Culture
denaturing gradient gel electrophoresis
DNA Primers
Electrophoresis, Polyacrylamide Gel
Exact sciences and technology
FAME
fatty acid methyl esters
Fatty acids
genes
Iron
microbial communities
Microorganisms
mixed culture
Perchlorate
Perchlorates
Perchlorates - metabolism
Phylogenetic analysis
Phylogeny
Pollution
Polymerase Chain Reaction
Principal Component Analysis
Receiving
Reduction
Zero-valent iron
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Summary:Anaerobic microbial mixed cultures demonstrated its ability to completely remove perchlorate in the presence of zero-valent iron. In order to understand the major microbial reaction in the iron-supported culture, community analysis comprising of microbial fatty acids and polymerase chain reaction–denaturing gradient gel electrophoresis (PCR–DGGE) techniques was performed for perchlorate reducing cultures. Analysis of fatty acid methyl esters (FAMEs) and subsequent principal component analysis (PCA) showed clear distinctions not only between iron-supported perchlorate reducing culture and seed bacteria, but also among perchlorate-reducing cultures receiving different electron donors. The DGGE pattern targeting the chlorite dismutase ( cld) gene showed that iron-supported perchlorate reducing culture is similar to hydrogen-fed cultures as compared to acetate-fed culture. The phylogenetic tree suggested that the dominant microbial reaction may be a combination of the autotrophic and heterotrophic reduction of perchlorate. Both molecular and chemotaxonomic experimental results support further understanding in the function of zero-valent iron as an adequate electron source for enhancing the microbial perchlorate reduction in natural and engineered systems.
ISSN:0304-3894
1873-3336
DOI:10.1016/j.jhazmat.2010.09.070