Novel lytic bacteriophages from soil that lyse Burkholderia pseudomallei

Burkholderia pseudomallei is a Gram-negative saprophytic bacterium that causes severe sepsis with a high mortality rate in humans and a vaccine is not available. Bacteriophages are viruses of bacteria that are ubiquitous in nature. Several lysogenic phages of Burkholderia spp. have been found but in...

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Bibliographic Details
Published in:FEMS microbiology letters 2011, Vol.314 (1), p.81-88
Main Authors: Yordpratum, Umaporn, Tattawasart, Unchalee, Wongratanacheewin, Surasakdi, Sermswan, Rasana W
Format: Article
Language:English
Subjects:
Aldehydes
Bacteria
Bacteriology
Bacteriolysis
Bacteriophages - classification
Bacteriophages - genetics
Bacteriophages - isolation & purification
Bacteriophages - physiology
biocontrol
Biological and medical sciences
Biological control
Burkholderia pseudomallei
Burkholderia pseudomallei - physiology
Burkholderia pseudomallei - virology
Fundamental and applied biological sciences. Psychology
Genomes
Host Specificity
Lysis
lytic phage
Microbiology
Miscellaneous
Multiplicity of infection
Phages
Plaques
Sepsis
soil
Soil Microbiology
Soil microorganisms
Soils
Virology
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Summary:Burkholderia pseudomallei is a Gram-negative saprophytic bacterium that causes severe sepsis with a high mortality rate in humans and a vaccine is not available. Bacteriophages are viruses of bacteria that are ubiquitous in nature. Several lysogenic phages of Burkholderia spp. have been found but information is scarce for lytic phages. Six phages, ST2, ST7, ST70, ST79, ST88 and ST96, which lyse B. pseudomallei, were isolated from soil in an endemic area. The phages belong to the Myoviridae family. The range of estimated genome sizes is 24.0-54.6 kb. Phages ST79 and ST96 lysed 71% and 67% of tested B. pseudomallei isolates and formed plaques on Burkholderia mallei but not other tested bacteria, with the exception of closely related Burkholderia thailandensis which was lysed by ST2 and ST96 only. ST79 and ST96 were observed to clear a mid-log culture by lysis within 6 h when infected at a multiplicity of infection of 0.1. As ST79 and ST96 phages effectively lysed B. pseudomallei, their potential use as a biocontrol of B. pseudomallei in the environment or alternative treatment in infected hosts could lead to benefits from phages that are available in nature.
ISSN:0378-1097
1574-6968
DOI:10.1111/j.1574-6968.2010.02150.x