Isolation and characterization of cynomolgus macaque ( Macaca fascicularis ) cytomegalovirus (CyCMV)

Abstract Cynomolgus macaques have been widely used as an animal model in preclinical biomedical research and are becoming more popular among HIV/SIV vaccine researchers. Here we report the isolation and characterization of a cytomegalovirus from cynomolgus macaques (CyCMV). CyCMV was isolated from a...

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Bibliographic Details
Published in:Virology (New York, N.Y.) N.Y.), 2011-03, Vol.412 (1), p.125-135
Main Authors: Ambagala, Aruna P.N, Marsh, Angie, Chan, Jacqueline, Pilon, Richard, Fournier, Jocelyn, Mazzulli, Tony, Sandstrom, Paul, Willer, David O, MacDonald, Kelly S
Format: Article
Language:English
Subjects:
Animals
Cluster Analysis
Cynomolgus macaques
Cytomegalovirus
Cytomegalovirus - genetics
Cytomegalovirus - isolation & purification
Cytomegalovirus - physiology
Cytomegalovirus - ultrastructure
DNA, Viral - chemistry
DNA, Viral - genetics
Glycoprotein B
Infectious Disease
Macaca fascicularis - virology
Molecular Sequence Data
Non-human primate
Phylogeny
Sequence Analysis, DNA
Sequence Homology, Amino Acid
Viral Proteins - genetics
Virus Cultivation
β-Herpesvirus
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Summary:Abstract Cynomolgus macaques have been widely used as an animal model in preclinical biomedical research and are becoming more popular among HIV/SIV vaccine researchers. Here we report the isolation and characterization of a cytomegalovirus from cynomolgus macaques (CyCMV). CyCMV was isolated from a healthy captive-bred 4-year-old cynomolgus macaque of Filipino origin. The virus was identified by its characteristic growth properties in cell culture, ultrastructural morphology and sequence of viral DNA polymerase and glycoprotein B (gB). CyCMV gB shows 77% identity and 88% homology to rhesus cytomegalovirus (RhCMV) gB and 58% identity and 76% homology to human cytomegalovirus gB at the amino acid level. Phylogenetic analysis using known CMV gB protein sequences show that CyCMV is more closely related to RhCMV than to other primate CMVs. CyCMV down-regulates MHC class I expression on infected cells and we show that the colony-bred cynomolgus macaques have detectable CyCMV-specific humoral and cell-mediated immune responses.
ISSN:0042-6822
1096-0341
DOI:10.1016/j.virol.2010.12.049