Dual Path Platform HIV 1/2 assay: evaluation of a novel rapid test using oral fluids for HIV screening at the National Hospital in Abuja, Nigeria

Abstract HIV/AIDS disease is endemic in Nigeria and associated with stigmatization. Availability of a reliable rapid testing kit and procedure will increase uptake of services. The study aimed to determine the correlation between detection of HIV antibodies in blood to that in oral fluid and to dete...

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Bibliographic Details
Published in:Diagnostic microbiology and infectious disease 2011-04, Vol.69 (4), p.405-409
Main Authors: Iregbu, Kenneth C, Esfandiari, Javan, Nnorom, Joseph, Sonibare, Samuel A, Uwaezuoke, Stella N, Eze, Stella O, Abdullahi, Nasiru, Lawal, Akeem O, Durogbola, Babatunde S
Format: Article
Language:English
Subjects:
Adolescent
Adult
Biological and medical sciences
Blotting, Western
Dual Path Pathway
Female
Fundamental and applied biological sciences. Psychology
HIV Antibodies - blood
HIV Infections - blood
HIV Infections - diagnosis
HIV Infections - immunology
HIV Infections - virology
HIV screening
HIV-1 - immunology
HIV-1 - isolation & purification
HIV-2 - immunology
HIV-2 - isolation & purification
Humans
Infectious Disease
Infectious diseases
Internal Medicine
Male
Medical sciences
Microbiology
Middle Aged
Miscellaneous
Mouth Mucosa - virology
National Hospital
Nigeria
Oral fluid
Reagent Kits, Diagnostic
Sensitivity and Specificity
Virology
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Summary:Abstract HIV/AIDS disease is endemic in Nigeria and associated with stigmatization. Availability of a reliable rapid testing kit and procedure will increase uptake of services. The study aimed to determine the correlation between detection of HIV antibodies in blood to that in oral fluid and to determine the sensitivity and specificity of the Dual Path Platform (DPP) testing kit using oral fluid samples. HIV antibodies detected in oral mucosa transudate and whole capillary blood from HIV-positive, high-risk and low-risk participants were compared with results obtained with whole venous blood from the same participants tested with Determine and Western blot (for discordant cases). Oral fluid test has sensitivity and specificity of 100% relative to Determine rapid assay, while whole capillary blood test has sensitivity of 100% and specificity of 99.5%. DPP oral fluid test is a reliable point-of-care test and may be deployed in large-scale screening exercises.
ISSN:0732-8893
1879-0070
DOI:10.1016/j.diagmicrobio.2010.10.011