Transplantation of olfactory ensheathing cells promotes axonal regeneration and functional recovery of peripheral nerve lesion in rats

Introduction: Olfactory ensheathing cells (OECs) hold promise for cell therapy because they may promote regeneration of the central nervous system. However, OECs have been less studied after peripheral nerve injury (PNI). The purpose of this investigation was to determine the effect of OEC transplan...

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Bibliographic Details
Published in:Muscle & nerve 2011-04, Vol.43 (4), p.543-551
Main Authors: Guérout, Nicolas, Duclos, Célia, Drouot, Laurent, Abramovici, Olivia, Bon-Mardion, Nicolas, Lacoume, Yann, Jean, Laetitia, Boyer, Olivier, Marie, Jean-Paul
Format: Article
Language:English
Subjects:
Animals
axonal regrowth
Axons - physiology
Biological and medical sciences
Cell Transplantation - methods
Cells, Cultured
Development. Senescence. Regeneration. Transplantation
Fundamental and applied biological sciences. Psychology
Male
Muscle Strength - physiology
Nerve Regeneration - physiology
neurotrophic factors
Olfactory Bulb - cytology
Olfactory Bulb - physiology
Olfactory Bulb - transplantation
olfactory ensheathing cells
peripheral nerve
Rats
Rats, Inbred F344
Recovery of Function - physiology
regeneration
Sciatic Neuropathy - pathology
Sciatic Neuropathy - surgery
Vertebrates: nervous system and sense organs
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Summary:Introduction: Olfactory ensheathing cells (OECs) hold promise for cell therapy because they may promote regeneration of the central nervous system. However, OECs have been less studied after peripheral nerve injury (PNI). The purpose of this investigation was to determine the effect of OEC transplantation on a severe sciatic nerve (SN) lesion. Methods: OECs were injected in rats after section and 2‐cm resection of the SN. Results: Three months after therapy, muscle strength and morphometric studies showed complete restoration of the contractile properties of the gastrocnemius and complete repair of the SN. Immunohistochemistry and RT‐PCR studies indicated an increase in the presence of neurotrophic factors. Interestingly, tracking of green fluorescent protein (GFP)‐positive OECs showed that no OECs were present in the SN. Discussion: Our results demonstrate that, after severe PNI, OECs have remarkable potential for nerve regeneration by creating a favorable microenvironment. Muscle Nerve, 2011
ISSN:0148-639X
1097-4598
DOI:10.1002/mus.21907