Ear Keloids as a Primary Candidate for the Application of Mitomycin C after Shave Excision: In Vivo and In Vitro Study

• Published in: Dermatologic surgery 2011-02, Vol.37 (2), p.168-175
• Main Authors: CHI, SEONG GEUN, KIM, JUN YOUNG, LEE, WEON JU, LEE, SEOK‐JONG, KIM, DO WON, SOHN, MI YEUNG, KIM, GUN WOOK, KIM, MOON BUM, KIM, BYUNG SOO
• Format: Article
• Language: English
• Subjects: Adolescent; Adult; Biological and medical sciences; Body Piercing - adverse effects; Cell Culture Techniques; Dermatology; Ear, External; Female; Fibroblasts - drug effects; Fibroblasts - pathology; Humans; Keloid - drug therapy; Keloid - etiology; Keloid - surgery; Male; Medical sciences; Middle Aged; Mitomycin - pharmacology; Mitomycin - therapeutic use; Nucleic Acid Synthesis Inhibitors - pharmacology; Nucleic Acid Synthesis Inhibitors - therapeutic use; Skin involvement in other diseases. Miscellaneous. General aspects; Skin plastic surgery; Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases; Young Adult
• ISSN: 1076-0512; 1524-4725
• DOI: 10.1111/j.1524-4725.2010.01846.x

BACKGROUND Although many methods have been developed to treat ear keloids, new therapeutic options are still needed. OBJECTIVE To determine the effects of topical mitomycin C (MC) on shave‐removed wounds and fibroblasts of ear keloids. METHODS Fourteen ear keloids in 12 patients were shaved, and MC (1 mg/mL) was applied to the resected bed for 5 minutes. The application was repeated 3 weeks later. All patients were assessed 2, 4, and 6 months after the procedure to evaluate the cosmetic results, recurrence, and postsurgical complications. An in vitro study to determine the effects of MC on fibroblasts of the excised keloids was conducted using the 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyltetrazolium bromide (MTT) assay, the measurement of total cell counts, and immunoassay of DNA synthesis. RESULTS Only one recurrence occurred (on the ear helix), and the patients were satisfied with the cosmetic outcomes. The results of the MTT assay, total cell counts, and DNA synthesis immunoassay confirmed the suppressive effects of MC on the keloid fibroblasts. CONCLUSION The application of topical MC to the resected bed of shave‐removed ear keloids was successful in preventing recurrences and providing an acceptable cosmetic outcome. The authors have indicated no significant interest with commercial supporters.