Cough reflex sensitivity is increased in guinea pigs with parainfluenza virus infection

• Published in: Experimental lung research 2011-04, Vol.37 (3), p.186-194
• Main Authors: Ye, X. M., Zhong, N. S., Liu, C. L., Chen, R. C.
• Format: Article
• Language: English
• Subjects: Animals; Base Sequence; Bronchial Hyperreactivity - etiology; Bronchial Hyperreactivity - physiopathology; Bronchoalveolar Lavage Fluid - cytology; Capsaicin; Chronic Disease; cough; Cough - etiology; Cough - physiopathology; cough reflex sensitivity; Disease Models, Animal; DNA Primers - genetics; Guinea Pigs; human parainfluenza virus type 3; Humans; Lung - pathology; Male; Parainfluenza Virus 3, Human - genetics; postinfectious cough; Reflex - physiology; Respirovirus Infections - complications; Respirovirus Infections - pathology; Respirovirus Infections - physiopathology; Respirovirus Infections - virology; RNA, Viral - genetics; RNA, Viral - metabolism; Time Factors
• ISSN: 0190-2148; 1521-0499
• DOI: 10.3109/01902148.2010.540768

ABSTRACT The purpose of this study was to investigate for the change in cough reflex sensitivity (CRS) caused by parainfluenza virus type 3 (PIV3) infection. Guinea pigs were randomized into a vehicle control, an asthma control, or 1 of 4 PIV3-inoculated groups (referred to as postinfection day [PID] 6, 12, 28, and 42 groups). Evidence of viral protein and nucleic acid within the lung confirmed successful PIV3 infection. Plethysmography was used to assess CRS and airway reaction and airway inflammation was assessed via bronchoalveolar lavage fluid cytology and lung histopathology. Compared with the vehicle control group, CRS was significantly increased in all PID groups (P