A novel capillary electrophoretic method for determining aliphatic aldehydes in food samples using 2-thiobarbituric acid derivatization

A novel electrophoretic method for sensitive determination of nine aldehydes, including formaldehyde (C1), acetaldehyde (C2), propanal (C3), butanal (C4), pentanal (C5), hexanal (C6), glutaradehyde (Gla), 2,3‐butanedione (Bud) and methylgloxal (MGo) in food samples, has been developed based on CE wi...

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Bibliographic Details
Published in:Electrophoresis 2011-03, Vol.32 (6-7), p.705-711
Main Authors: Zhang, Jun-Bo, Li, Meng-Jie, Li, Wen-Li, Chu, Qing-Cui, Ye, Jian-Nong
Format: Article
Language:English
Subjects:
2-Thiobarbituric acid
Acetaldehyde
Aldehyde
Aldehydes
Aldehydes - analysis
Aldehydes - chemistry
Aliphatic compounds
Amperometric detection
Calibration
Capillary electrophoresis
Conferences
Dietary Fats
Electrophoresis, Capillary - methods
Food Analysis - methods
Foods
Hydrogen-Ion Concentration
Linear Models
Magnesium oxide
Mathematical analysis
Reproducibility of Results
Seeds
Sensitivity and Specificity
Temperature
Thiobarbiturates - chemistry
Wine
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Summary:A novel electrophoretic method for sensitive determination of nine aldehydes, including formaldehyde (C1), acetaldehyde (C2), propanal (C3), butanal (C4), pentanal (C5), hexanal (C6), glutaradehyde (Gla), 2,3‐butanedione (Bud) and methylgloxal (MGo) in food samples, has been developed based on CE with amperometric detection (CE‐AD). After being derivatized with an electroactive compound, 2‐thiobarbituric acid (TBA), these nine non‐electroactive aldehydes were converted to electroactive adducts, and therefore detectable by CE‐AD approach. Experimental conditions of derivatization and CE‐AD detection were optimized. The proposed method was validated according to International Conference on Harmonization (ICH) requirements, with recovery results ranging from 82.8 to 123.8%. Calibration plots of aliphatic aldehydes were linear (r2≥0.9901) in the concentration range from 0.083 to 15.0 mg/L. The LODs were between 0.008 and 0.074 mg/L. The proposed CE‐AD method provides a reliable and sensitive quantitative evaluation for non‐electroactive low‐molecular‐mass monoaldehydes and dialdehydes in real sample matrices by employing relatively simple and inexpensive instrument.
ISSN:0173-0835
1522-2683
DOI:10.1002/elps.201000533