Characterization of Vibrio tapetis strains isolated from diseased cultured Wedge sole ( Dicologoglossa cuneata Moreau)

The first isolation of Vibrio tapetis from Wedge sole ( Dicologoglossa cuneata) is reported. The bacterium was recovered from ulcers of ailing cultured fish, from two different outbreaks occurred in spring 2005. The four isolates found (a200, a201, a204 and a255) were biochemically, genetically and...

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Bibliographic Details
Published in:Research in veterinary science 2011-04, Vol.90 (2), p.189-195
Main Authors: López, J.R., Balboa, S., Núñez, S., de la Roca, E., de la Herran, R., Navas, J.I., Toranzo, A.E., Romalde, J.L.
Format: Article
Language:English
Subjects:
Acids
Animals
Aquaculture
Characterization
Dicologoglossa cuneata
Fish
Fish Diseases - microbiology
Flatfishes
Glucose
Lactose
Microbiology
Phylogeny
Salinity
Seawater
Sucrose
Veterinary medicine
Vibrio - classification
Vibrio - genetics
Vibrio - isolation & purification
Vibrio Infections - microbiology
Vibrio Infections - veterinary
Vibrio tapetis
Virulence
Wedge sole
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Summary:The first isolation of Vibrio tapetis from Wedge sole ( Dicologoglossa cuneata) is reported. The bacterium was recovered from ulcers of ailing cultured fish, from two different outbreaks occurred in spring 2005. The four isolates found (a200, a201, a204 and a255) were biochemically, genetically and serologically characterized and diagnosis was confirmed by PCR V. tapetis specific primers and multilocus sequencing analysis (MLSA). The isolates constituted a homogeneous phenotypic and genotypic group, being distinct to the already serological and genetic groups defined within the species. A virulence evaluation of the isolate a255 was also carried out; however this strain was unable to induce disease in fry and juvenile Wedge sole.
ISSN:0034-5288
1532-2661
DOI:10.1016/j.rvsc.2010.05.030