Biotechniques Transfection of cells by immunoporation

Cell transfection is now a central technique in molecular biology and an essential prerequisite for gene therapy. Here we describe how beads coated with antibodies and bound to specific cell-surface transmembrane proteins can create holes in cells when the beads are removed, allowing transfection of...

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Bibliographic Details
Published in:Nature (London) 2000-05, Vol.405 (6784), p.298-298
Main Authors: Rickwood, David, Bildirici, Lale, Smith, Patricia, Tzavelas, Christos, Horefti, Elina
Format: Article
Language:English
Subjects:
Animal communication
Antibodies
Antigens
Cell death
Flow cytometry
Gene therapy
Molecular biology
Online Access:Get full text
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Summary:Cell transfection is now a central technique in molecular biology and an essential prerequisite for gene therapy. Here we describe how beads coated with antibodies and bound to specific cell-surface transmembrane proteins can create holes in cells when the beads are removed, allowing transfection of the cells with DNA or other macromolecules. This unique targeted transfection of cells by immunoporation is very efficient and results in minimal cell death.
ISSN:0028-0836
1476-4687
DOI:10.1038/35012701