Influence of selected antibiotics on the response of black fly (Simulium vittatum) larvae to insecticidal proteins produced by Bacillus thuringiensis subsp. israelensis

• Published in: Environmental toxicology and chemistry 2010-08, Vol.29 (8), p.1849-1853
• Main Authors: Iburg, Joseph P., Gray, Elmer W., Wyatt, Roger D., Noblet, Raymond
• Format: Article
• Language: English
• Subjects: Animals; Anthropogenic factors; Anti-Bacterial Agents - chemistry; Anti-Bacterial Agents - toxicity; Antibiotics; Bacillus thuringiensis; Bacillus thuringiensis subsp. israelensis; Bacterial Proteins - chemistry; Bacterial Proteins - toxicity; Black flies; Black fly larvae; Contamination; Diptera; Dose-Response Relationship, Drug; Drug Interactions; Effectiveness; Endotoxins - chemistry; Endotoxins - toxicity; Exposure; Gram-positive bacteria; Hemolysin Proteins - chemistry; Hemolysin Proteins - toxicity; Inductively coupled plasma; Insecticides - chemistry; Insecticides - toxicity; Insects; Larva - drug effects; Larvae; Proteins; Rivers; Simuliidae - drug effects; Simulium vitattum; Simulium vittatum; Toxicity; Toxicology; Water Pollutants, Chemical - chemistry; Water Pollutants, Chemical - toxicity; Water pollution; Waterways
• ISSN: 0730-7268; 1552-8618; 1552-8618
• DOI: 10.1002/etc.225

A controlled current toxicity test (CCTT) was developed to evaluate the response of black fly (Simulium vittatum) larvae to insecticidal proteins following exposure to various antibiotics. The bacterium, Bacillus thuringiensis subsp. israelensis (Bti), produces proteins that are toxic to Nemotoceran Diptera, such as black flies and mosquitoes, when ingested. These insecticidal crystalline proteins (ICPs) are highly efficacious in controlling black flies; however, speculation has arisen regarding the potential for antibiotic contamination of waterways to mitigate the toxicity of these proteins. A series of experiments was conducted with the CCTT in which black fly larvae were exposed to enrofloxacin, tylosin, sulfamethoxazole, and trimethoprim followed by exposure to Bti ICPs. These antibiotics were selected based on their use in agricultural and documented anthropogenic contamination of rivers. Anthropogenic concentrations of a mixture of these four antibiotics did not affect the response of the larvae to Bti ICPs. Subsequent experiments were conducted with antibiotic concentrations 10,000 to 80,000 times higher than those found in contaminated rivers. Exposure of black fly larvae to high levels of enrofloxacin (0.5 mg/L) had no effect upon the susceptibility to Bti ICPs; however, exposure to high levels of tylosin (8 mg/L) resulted in a significant increase in the susceptibility of the larvae to Bti ICPs at 72 h of exposure, but not at 48 h. Exposure of black fly larvae to high concentrations of a mixture of sulfamethoxazole and trimethoprim resulted in a significant increase in the efficacy of the larvicide after 48 and 72 h of exposure. These results suggest that impairment of the efficacy of Bti ICPs to black fly larvae is not due to antibiotic contamination of the larval environment. Environ. Toxicol. Chem. 2010; 29:1849–1853. © 2010 SETAC