Molecular impact and inducible factors associated with adenovirus infection in hematopoietic stem [corrected] cell transplant patients

• Published in: Transplantation proceedings 2011-03, Vol.43 (2), p.644-646
• Main Authors: Yaghobi, R, Langari, M J, Ramzi, M, Geramizadeh, B, Zakerinia, M, Khojasteh, H N, Dehghani, M
• Format: Article
• Language: English
• Subjects: Adenoviridae - genetics; Adenoviridae Infections - diagnosis; Adolescent; Adult; Child; Cross-Sectional Studies; Edetic Acid - chemistry; Female; Genome, Viral; Graft vs Host Disease; Hematopoietic Stem Cells - cytology; Humans; Male; Polymerase Chain Reaction; Skin - virology; Skin Transplantation - methods
• ISSN: 1873-2623
• DOI: 10.1016/j.transproceed.2011.01.028

Latent and active adenovirus infections are detected in 5% to 20% of hematopoietic stem cell transplant (HSCT) patients. In addition to the significant role of adenoviral infection in the pathogenesis of late-onset hemorrhagic cystitis in HSCT patients, adenovirus infections may have possible roles in undefined posttransplant clinical complications. Therefore, pre- and posttransplantation we studied the prevalence and role of adenoviral infections among HSCT clinical syndromes using molecular methods. In this cross-sectional study between 2005 and 2008. We collected 470 EDTA-treated blood samples from 125 HSCT recipients, including 70 (56%) men and 55 (44%) women. The 52 (41.6%) HSCT patients underwent autologous grafts and the other 73 (58.4%), from related donors. One EDTA-treated blood sample was collected from all recipients pretransplantation. Also once per week for 3 months we were collected blood samples from HSCT patients to evaluate the prevalence of adenovirus DNA infection by a qualitative in house polymerase chain reaction method. The adenovirus genome was diagnosed in 2/75 (2.7%) HSCT patient samples pretransplantation. There were 28/395 (7.1%) plasma samples of transplant patients infected with adenovirus DNA. Graft-versus-host disease (GVHD) clinical complications were observed in six adenovirus-infected transplant recipients; there was a significant correlation between these viral infections and GVHD clinical presentation. The high prevalence of adenovirus infection in HSCT recipients pre- and posttransplantation, was significantly related to GVHD symptoms, enforcing the important pathogenic role of these viral infections in clinical complications post-HSCT.