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Cloning and characterization of two new Class III peroxidase genes from Catharanthus roseus
We report here the cloning and characterization of two new class III peroxidase genes, CrPrx3 and CrPrx4 from Catharanthus roseus. The full length cDNA of CrPrx3 is 1233 bp long encoding 330 amino acid residues. The CrPrx4 cDNA contains an ORF of 1055 bp, and encodes for 318 amino acids. Hypothetica...
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| Published in: | Plant physiology and biochemistry 2011-04, Vol.49 (4), p.404-412 |
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| container_issue | 4 |
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| container_title | Plant physiology and biochemistry |
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| creator | Kumar, Santosh Jaggi, Monika Taneja, Jyoti Sinha, Alok Krishna |
| description | We report here the cloning and characterization of two new class III peroxidase genes,
CrPrx3 and
CrPrx4 from
Catharanthus roseus. The full length cDNA of
CrPrx3 is 1233 bp long encoding 330 amino acid residues. The
CrPrx4 cDNA contains an ORF of 1055 bp, and encodes for 318 amino acids. Hypothetical 3-D structure model of
CrPrx3 and
CrPrx4 detected the presence of two Ca
2+ ion binding sites at proximal and distal end, and a heme group coordinate at central position.
CrPrx3 and
CrPrx4 are present in single copy number in
C. roseus. Confocal scanning microscopy and phylogenetic analysis showed that
CrPrx3 and
CrPrx4 are apoplastic in nature. Phylogenetic analysis revealed that both genes have diverse expression patterns in a variety of plant tissues. Quantitative real-time PCR confirmed their maximum expression in stem tissues followed by flower tissue. The expression levels of
CrPrx3 and
CrPrx4 were down-regulated under salt and dehydration stress, respectively.
► Cloning and characterization of two new peroxidases of
Catharanthus roseus is reported. ► Both peroxidases
CrPrx3 and
CrPrx4 are down-regulated under salt and dehydration stress. ► qRT-PCR data indicate their differential expression pattern in tissue specific manner. |
| doi_str_mv | 10.1016/j.plaphy.2011.01.012 |
| format | article |
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CrPrx3 and
CrPrx4 from
Catharanthus roseus. The full length cDNA of
CrPrx3 is 1233 bp long encoding 330 amino acid residues. The
CrPrx4 cDNA contains an ORF of 1055 bp, and encodes for 318 amino acids. Hypothetical 3-D structure model of
CrPrx3 and
CrPrx4 detected the presence of two Ca
2+ ion binding sites at proximal and distal end, and a heme group coordinate at central position.
CrPrx3 and
CrPrx4 are present in single copy number in
C. roseus. Confocal scanning microscopy and phylogenetic analysis showed that
CrPrx3 and
CrPrx4 are apoplastic in nature. Phylogenetic analysis revealed that both genes have diverse expression patterns in a variety of plant tissues. Quantitative real-time PCR confirmed their maximum expression in stem tissues followed by flower tissue. The expression levels of
CrPrx3 and
CrPrx4 were down-regulated under salt and dehydration stress, respectively.
► Cloning and characterization of two new peroxidases of
Catharanthus roseus is reported. ► Both peroxidases
CrPrx3 and
CrPrx4 are down-regulated under salt and dehydration stress. ► qRT-PCR data indicate their differential expression pattern in tissue specific manner.</description><identifier>ISSN: 0981-9428</identifier><identifier>EISSN: 1873-2690</identifier><identifier>DOI: 10.1016/j.plaphy.2011.01.012</identifier><identifier>PMID: 21320785</identifier><identifier>CODEN: PPBIEX</identifier><language>eng</language><publisher>Paris: Elsevier Masson SAS</publisher><subject>Abiotic stress ; Adaptation, Physiological - genetics ; Amino Acid Sequence ; amino acids ; binding sites ; Biological and medical sciences ; Catharanthus - chemistry ; Catharanthus - genetics ; Catharanthus - metabolism ; Catharanthus roseus ; Cloning, Molecular ; complementary DNA ; copy number ; Dehydration ; Desiccation ; DNA, Complementary - analysis ; Down-Regulation ; Flowers ; Fundamental and applied biological sciences. Psychology ; Gene Expression ; Gene Expression Regulation, Plant ; genes ; Genes, Plant ; Heme ; Microscopy ; Models, Molecular ; Molecular Sequence Data ; Molecular Structure ; Nucleotides - analysis ; Open reading frames ; Peroxidase ; Peroxidase - chemistry ; Peroxidase - genetics ; Peroxidase - metabolism ; Peroxidases ; Phylogeny ; Plant physiology and development ; Plant Proteins - chemistry ; Plant Proteins - genetics ; Plant Proteins - metabolism ; Plant Stems ; plant tissues ; Polymerase Chain Reaction ; quantitative polymerase chain reaction ; Salt-Tolerance - genetics ; Salts ; Scanning ; Stress ; Stress, Physiological - genetics ; Sub-cellular localization</subject><ispartof>Plant physiology and biochemistry, 2011-04, Vol.49 (4), p.404-412</ispartof><rights>2011 Elsevier Masson SAS</rights><rights>2015 INIST-CNRS</rights><rights>Copyright © 2011 Elsevier Masson SAS. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c448t-a8517b44febe03be7c80bd06dfdb790c8e608dbc92e486c821fe91f5c8fbc1323</citedby><cites>FETCH-LOGICAL-c448t-a8517b44febe03be7c80bd06dfdb790c8e608dbc92e486c821fe91f5c8fbc1323</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=24037280$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21320785$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kumar, Santosh</creatorcontrib><creatorcontrib>Jaggi, Monika</creatorcontrib><creatorcontrib>Taneja, Jyoti</creatorcontrib><creatorcontrib>Sinha, Alok Krishna</creatorcontrib><title>Cloning and characterization of two new Class III peroxidase genes from Catharanthus roseus</title><title>Plant physiology and biochemistry</title><addtitle>Plant Physiol Biochem</addtitle><description>We report here the cloning and characterization of two new class III peroxidase genes,
CrPrx3 and
CrPrx4 from
Catharanthus roseus. The full length cDNA of
CrPrx3 is 1233 bp long encoding 330 amino acid residues. The
CrPrx4 cDNA contains an ORF of 1055 bp, and encodes for 318 amino acids. Hypothetical 3-D structure model of
CrPrx3 and
CrPrx4 detected the presence of two Ca
2+ ion binding sites at proximal and distal end, and a heme group coordinate at central position.
CrPrx3 and
CrPrx4 are present in single copy number in
C. roseus. Confocal scanning microscopy and phylogenetic analysis showed that
CrPrx3 and
CrPrx4 are apoplastic in nature. Phylogenetic analysis revealed that both genes have diverse expression patterns in a variety of plant tissues. Quantitative real-time PCR confirmed their maximum expression in stem tissues followed by flower tissue. The expression levels of
CrPrx3 and
CrPrx4 were down-regulated under salt and dehydration stress, respectively.
► Cloning and characterization of two new peroxidases of
Catharanthus roseus is reported. ► Both peroxidases
CrPrx3 and
CrPrx4 are down-regulated under salt and dehydration stress. ► qRT-PCR data indicate their differential expression pattern in tissue specific manner.</description><subject>Abiotic stress</subject><subject>Adaptation, Physiological - genetics</subject><subject>Amino Acid Sequence</subject><subject>amino acids</subject><subject>binding sites</subject><subject>Biological and medical sciences</subject><subject>Catharanthus - chemistry</subject><subject>Catharanthus - genetics</subject><subject>Catharanthus - metabolism</subject><subject>Catharanthus roseus</subject><subject>Cloning, Molecular</subject><subject>complementary DNA</subject><subject>copy number</subject><subject>Dehydration</subject><subject>Desiccation</subject><subject>DNA, Complementary - analysis</subject><subject>Down-Regulation</subject><subject>Flowers</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression</subject><subject>Gene Expression Regulation, Plant</subject><subject>genes</subject><subject>Genes, Plant</subject><subject>Heme</subject><subject>Microscopy</subject><subject>Models, Molecular</subject><subject>Molecular Sequence Data</subject><subject>Molecular Structure</subject><subject>Nucleotides - analysis</subject><subject>Open reading frames</subject><subject>Peroxidase</subject><subject>Peroxidase - chemistry</subject><subject>Peroxidase - genetics</subject><subject>Peroxidase - metabolism</subject><subject>Peroxidases</subject><subject>Phylogeny</subject><subject>Plant physiology and development</subject><subject>Plant Proteins - chemistry</subject><subject>Plant Proteins - genetics</subject><subject>Plant Proteins - metabolism</subject><subject>Plant Stems</subject><subject>plant tissues</subject><subject>Polymerase Chain Reaction</subject><subject>quantitative polymerase chain reaction</subject><subject>Salt-Tolerance - genetics</subject><subject>Salts</subject><subject>Scanning</subject><subject>Stress</subject><subject>Stress, Physiological - genetics</subject><subject>Sub-cellular localization</subject><issn>0981-9428</issn><issn>1873-2690</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><recordid>eNp90U1v1DAQBmALgei28A8Q-ILKJcvYcRLngoQiPlaqxAF64mA5znjXq2wc7IRSfj2OssCtkiVfnvF45iXkBYMtA1a-PW7HXo-H-y0HxrawHP6IbJis8oyXNTwmG6gly2rB5QW5jPEIAFxU-VNywVnOoZLFhnxvej-4YU_10FFz0EGbCYP7rSfnB-otne48HfCONr2Oke52Ozpi8L9cpyPSPQ4YqQ3-RBs9LdXDdJgjDT7iHJ-RJ1b3EZ-f7yty-_HDt-ZzdvPl0655f5MZIeSUaVmwqhXCYouQt1gZCW0HZWe7tqrBSCxBdq2pOQpZGsmZxZrZwkjbmjRIfkWu13fH4H_MGCd1ctFg3-sB_RyVLGpes7xc5JsHJQPOizIvmUxUrNSkYWJAq8bgTjrcJ6SWANRRrQGoJQAFy1k6vDx3mNsTdv-K_m48gddnoKPRvU0rMy7-dwLyiktI7tXqrPZK70Myt19TJ5FSTH3KOol3q8C0258Og4rG4WCwcwHNpDrvHv7rH2Glr3Q</recordid><startdate>20110401</startdate><enddate>20110401</enddate><creator>Kumar, Santosh</creator><creator>Jaggi, Monika</creator><creator>Taneja, Jyoti</creator><creator>Sinha, Alok Krishna</creator><general>Elsevier Masson SAS</general><general>Elsevier</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20110401</creationdate><title>Cloning and characterization of two new Class III peroxidase genes from Catharanthus roseus</title><author>Kumar, Santosh ; Jaggi, Monika ; Taneja, Jyoti ; Sinha, Alok Krishna</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c448t-a8517b44febe03be7c80bd06dfdb790c8e608dbc92e486c821fe91f5c8fbc1323</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Abiotic stress</topic><topic>Adaptation, Physiological - genetics</topic><topic>Amino Acid Sequence</topic><topic>amino acids</topic><topic>binding sites</topic><topic>Biological and medical sciences</topic><topic>Catharanthus - chemistry</topic><topic>Catharanthus - genetics</topic><topic>Catharanthus - metabolism</topic><topic>Catharanthus roseus</topic><topic>Cloning, Molecular</topic><topic>complementary DNA</topic><topic>copy number</topic><topic>Dehydration</topic><topic>Desiccation</topic><topic>DNA, Complementary - analysis</topic><topic>Down-Regulation</topic><topic>Flowers</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Expression</topic><topic>Gene Expression Regulation, Plant</topic><topic>genes</topic><topic>Genes, Plant</topic><topic>Heme</topic><topic>Microscopy</topic><topic>Models, Molecular</topic><topic>Molecular Sequence Data</topic><topic>Molecular Structure</topic><topic>Nucleotides - analysis</topic><topic>Open reading frames</topic><topic>Peroxidase</topic><topic>Peroxidase - chemistry</topic><topic>Peroxidase - genetics</topic><topic>Peroxidase - metabolism</topic><topic>Peroxidases</topic><topic>Phylogeny</topic><topic>Plant physiology and development</topic><topic>Plant Proteins - chemistry</topic><topic>Plant Proteins - genetics</topic><topic>Plant Proteins - metabolism</topic><topic>Plant Stems</topic><topic>plant tissues</topic><topic>Polymerase Chain Reaction</topic><topic>quantitative polymerase chain reaction</topic><topic>Salt-Tolerance - genetics</topic><topic>Salts</topic><topic>Scanning</topic><topic>Stress</topic><topic>Stress, Physiological - genetics</topic><topic>Sub-cellular localization</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kumar, Santosh</creatorcontrib><creatorcontrib>Jaggi, Monika</creatorcontrib><creatorcontrib>Taneja, Jyoti</creatorcontrib><creatorcontrib>Sinha, Alok Krishna</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Plant physiology and biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kumar, Santosh</au><au>Jaggi, Monika</au><au>Taneja, Jyoti</au><au>Sinha, Alok Krishna</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cloning and characterization of two new Class III peroxidase genes from Catharanthus roseus</atitle><jtitle>Plant physiology and biochemistry</jtitle><addtitle>Plant Physiol Biochem</addtitle><date>2011-04-01</date><risdate>2011</risdate><volume>49</volume><issue>4</issue><spage>404</spage><epage>412</epage><pages>404-412</pages><issn>0981-9428</issn><eissn>1873-2690</eissn><coden>PPBIEX</coden><abstract>We report here the cloning and characterization of two new class III peroxidase genes,
CrPrx3 and
CrPrx4 from
Catharanthus roseus. The full length cDNA of
CrPrx3 is 1233 bp long encoding 330 amino acid residues. The
CrPrx4 cDNA contains an ORF of 1055 bp, and encodes for 318 amino acids. Hypothetical 3-D structure model of
CrPrx3 and
CrPrx4 detected the presence of two Ca
2+ ion binding sites at proximal and distal end, and a heme group coordinate at central position.
CrPrx3 and
CrPrx4 are present in single copy number in
C. roseus. Confocal scanning microscopy and phylogenetic analysis showed that
CrPrx3 and
CrPrx4 are apoplastic in nature. Phylogenetic analysis revealed that both genes have diverse expression patterns in a variety of plant tissues. Quantitative real-time PCR confirmed their maximum expression in stem tissues followed by flower tissue. The expression levels of
CrPrx3 and
CrPrx4 were down-regulated under salt and dehydration stress, respectively.
► Cloning and characterization of two new peroxidases of
Catharanthus roseus is reported. ► Both peroxidases
CrPrx3 and
CrPrx4 are down-regulated under salt and dehydration stress. ► qRT-PCR data indicate their differential expression pattern in tissue specific manner.</abstract><cop>Paris</cop><pub>Elsevier Masson SAS</pub><pmid>21320785</pmid><doi>10.1016/j.plaphy.2011.01.012</doi><tpages>9</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0981-9428 |
| ispartof | Plant physiology and biochemistry, 2011-04, Vol.49 (4), p.404-412 |
| issn | 0981-9428 1873-2690 |
| language | eng |
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| source | ScienceDirect Freedom Collection |
| subjects | Abiotic stress Adaptation, Physiological - genetics Amino Acid Sequence amino acids binding sites Biological and medical sciences Catharanthus - chemistry Catharanthus - genetics Catharanthus - metabolism Catharanthus roseus Cloning, Molecular complementary DNA copy number Dehydration Desiccation DNA, Complementary - analysis Down-Regulation Flowers Fundamental and applied biological sciences. Psychology Gene Expression Gene Expression Regulation, Plant genes Genes, Plant Heme Microscopy Models, Molecular Molecular Sequence Data Molecular Structure Nucleotides - analysis Open reading frames Peroxidase Peroxidase - chemistry Peroxidase - genetics Peroxidase - metabolism Peroxidases Phylogeny Plant physiology and development Plant Proteins - chemistry Plant Proteins - genetics Plant Proteins - metabolism Plant Stems plant tissues Polymerase Chain Reaction quantitative polymerase chain reaction Salt-Tolerance - genetics Salts Scanning Stress Stress, Physiological - genetics Sub-cellular localization |
| title | Cloning and characterization of two new Class III peroxidase genes from Catharanthus roseus |
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