Coronary vein infusion of multipotent stromal cells from bone marrow preserves cardiac function in swine ischemic cardiomyopathy via enhanced neovascularization

• Published in: Laboratory investigation 2011-04, Vol.91 (4), p.553-564
• Main Authors: Sato, Takatoshi, Iso, Yoshitaka, Uyama, Taro, Kawachi, Keisuke, Wakabayashi, Kohei, Omori, Yasutoshi, Soda, Teruko, Shoji, Makoto, Koba, Shinji, Yokoyama, Shin-Ichiro, Fukuda, Noboru, Saito, Satoshi, Katagiri, Takashi, Kobayashi, Youichi, Takeyama, Youichi, Umezawa, Akihiro, Suzuki, Hiroshi
• Format: Article
• Language: English
• Subjects: 631/250/1620/1342; 631/443/592/16; 692/699/75/2/1674; 692/700/565/2319; angiogenesis; Animals; Biological and medical sciences; Biotechnology; bone marrow; Bone Marrow Transplantation - methods; Cardiology. Vascular system; Cell Differentiation; Chronic Disease; chronic myocardial infarction; coronary vein; Coronary Vessels; Fibroblast Growth Factor 2 - metabolism; Fibrosis; Fundamental and applied biological sciences. Psychology; Heart; Heart - physiopathology; Infusions, Intravenous; Investigative techniques, diagnostic techniques (general aspects); Laboratory Medicine; Medical sciences; Medicine; Medicine & Public Health; Multipotent Stem Cells - metabolism; Multipotent Stem Cells - pathology; Multipotent Stem Cells - transplantation; multipotent stromal cells; Myocardial Infarction - complications; Myocardial Ischemia - etiology; Myocardial Ischemia - pathology; Myocardial Ischemia - physiopathology; Myocardial Ischemia - surgery; Myocarditis. Cardiomyopathies; Myocardium - pathology; Neovascularization, Physiologic; Pathology; Phenotype; research-article; Stromal Cells - metabolism; Stromal Cells - pathology; Stromal Cells - transplantation; Swine; Vascular Endothelial Growth Factor A - metabolism
• ISSN: 0023-6837; 1530-0307
• DOI: 10.1038/labinvest.2010.202

Few reports have examined the effects of adult bone marrow multipotent stromal cells (MSCs) on large animals, and no useful method has been established for MSC implantation. In this study, we investigate the effects of MSC infusion from the coronary vein in a swine model of chronic myocardial infarction (MI). MI was induced in domestic swine by placing beads in the left coronary artery. Bone marrow cells were aspirated and then cultured to isolate the MSCs. At 4 weeks after MI, MSCs labeled with dye (n=8) or vehicle (n=5) were infused retrogradely from the anterior interventricular vein without any complications. Left ventriculography (LVG) was performed just before and at 4 weeks after cell infusion. The ejection fraction (EF) assessed by LVG significantly decreased from baseline up to a follow-up at 4 weeks in the control group (P