Aluminum-tolerant Pseudomonas fluorescens: ROS toxicity and enhanced NADPH production

Aluminum (Al) triggered a marked increase in reactive oxygen species (ROS) such as O(2) (-) and H(2)O(2) in Pseudomonas fluorescens. Although the Al-stressed cells were characterized with higher amounts of oxidized lipids and proteins than controls, NADPH production was markedly increased in these c...

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Published in:Extremophiles : life under extreme conditions 2005-10, Vol.9 (5), p.367-373
Main Authors: Singh, Ranji, Beriault, Robin, Middaugh, Jeffrey, Hamel, Robert, Chenier, Daniel, Appanna, Vasu D, Kalyuzhnyi, Sergey
Format: Article
Language:English
Subjects:
Aluminum
Aluminum - pharmacology
Bacteria
Cell Count
Dehydrogenase
Glucosephosphate Dehydrogenase - metabolism
Hydrogen peroxide
Hydrogen Peroxide - metabolism
Hydrogen Peroxide - toxicity
Isocitrate Dehydrogenase - metabolism
Lipids
NADP - biosynthesis
Oxidation-Reduction
Pseudomonas fluorescens
Pseudomonas fluorescens - cytology
Pseudomonas fluorescens - drug effects
Pseudomonas fluorescens - metabolism
Reactive Oxygen Species - metabolism
Reactive Oxygen Species - toxicity
Superoxides - metabolism
Superoxides - toxicity
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Summary:Aluminum (Al) triggered a marked increase in reactive oxygen species (ROS) such as O(2) (-) and H(2)O(2) in Pseudomonas fluorescens. Although the Al-stressed cells were characterized with higher amounts of oxidized lipids and proteins than controls, NADPH production was markedly increased in these cells. Blue native polyacrylamide gel electrophoresis (BN-PAGE) analyses coupled with activity and Coomassie staining revealed that NADP(+) -dependent isocitrate dehydrogenase (ICDH, E.C. 1.1.1.42) and glucose-6-phosphate dehydrogenase (G6PDH, E.C. 1.1.1.49) played a pivotal role in diminishing the oxidative environment promoted by Al. These enzymes were overexpressed in the Al-tolerant microbes and were modulated by the presence of either Al or hydrogen peroxide (H(2)O(2)) or menadione. The activity of superoxide dismutase (SOD, E.C. 1.15.1.1), an enzyme known to combat ROS stress was also increased in the cells cultured in millimolar amounts of Al. Hence, Al-tolerant P. fluorescens invokes an anti-oxidative defense strategy in order to survive.
ISSN:1431-0651
1433-4909
DOI:10.1007/s00792-005-0450-7