Improvement of substrate conversion to molecular hydrogen by three-stage cultivation of a photosynthetic bacterium, Rhodovulum sulfidophilum

In photosynthetic bacteria, after transition to light-anaerobic and nitrogen-deficient conditions, hydrogen evolution starts with expression of nitrogenase activity. Until the expression of enough activity, Rhodovulum sulfidophilum consumed substrates and converted them to poly(3-hydroxybutyrate) (P...

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Bibliographic Details
Published in:Applied biochemistry and biotechnology 1998-03, Vol.70 (1), p.301-310
Main Authors: Maeda, I. (Osaka University, Suita, Osaka, Japan.), Chowdhury, W.O, Idehara, K, Yagi, K, Mizoguchi, T, Akano, T, Miyasaka, H, Furutani, T, Ikuta, Y, Shioji, N
Format: Article
Language:English
Subjects:
Algae
Anaerobic conditions
BACTERIA
Biological and medical sciences
Biotechnology
Conversion
Cultivation
Derepression
Fundamental and applied biological sciences. Psychology
HIDROGENO
HYDROGEN
Hydrogen evolution
Hydrogen production
HYDROGENE
Lag time
Methods. Procedures. Technologies
Microbial engineering. Fermentation and microbial culture technology
NITROGENASA
NITROGENASE
Photosynthesis
Substrates
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Summary:In photosynthetic bacteria, after transition to light-anaerobic and nitrogen-deficient conditions, hydrogen evolution starts with expression of nitrogenase activity. Until the expression of enough activity, Rhodovulum sulfidophilum consumed substrates and converted them to poly(3-hydroxybutyrate) (PHB), resulting in a decrease in the proportion of substrate converted into hydrogen gas. To prevent conversion to PHB during the period when nitrogenase activity is derepressed, the authors employed a cultivation method consisting of three stages: cell growth, nitrogenase derepression, and hydrogen production. Cells cultivated by this method exhibited no lag time before the commencement of hydrogen evolution and gave an improved yield of hydrogen from the algal fermentative products
ISSN:0273-2289
1559-0291
DOI:10.1007/BF02920146