Infection with Specific Helicobacter pylori-cag Pathogenicity Island Strains Is Associated with Interleukin-1B Gene Polymorphisms in Venezuelan Chronic Gastritis Patients

• Published in: Digestive diseases and sciences 2011-02, Vol.56 (2), p.449-456
• Main Authors: Chiurillo, Miguel Angel, Moran, Yeinmy H, Cañas, Miryan, Valderrama, Elvis J, Armanie, Emma
• Format: Article
• Language: English
• Subjects: Adolescent; Adult; Aged; Aged, 80 and over; Antigens, Bacterial - metabolism; Bacterial diseases; Bacterial diseases of the digestive system and abdomen; Bacterial Proteins - metabolism; Biochemistry; Biological and medical sciences; Chronic Disease; Feeding. Feeding behavior; Female; Fundamental and applied biological sciences. Psychology; Gastritis; Gastritis - epidemiology; Gastritis - genetics; Gastritis - microbiology; Gastroenterology; Gastroenterology. Liver. Pancreas. Abdomen; Genes; Genetic aspects; Genetic polymorphisms; Genetic Predisposition to Disease; Health aspects; Helicobacter pylori; Helicobacter pylori - classification; Helicobacter pylori - metabolism; Hepatology; Human bacterial diseases; Humans; Infection; Infectious diseases; Interleukin-1beta - genetics; Interleukin-1beta - metabolism; Interleukins; Male; Medical sciences; Medicine; Medicine & Public Health; Middle Aged; Oncology; Original Article; Other diseases. Semiology; Polymorphism, Genetic; Stomach cancer; Stomach. Duodenum. Small intestine. Colon. Rectum. Anus; Transplant Surgery; Venezuela - epidemiology; Vertebrates: anatomy and physiology, studies on body, several organs or systems; Young Adult
• ISSN: 0163-2116; 1573-2568
• DOI: 10.1007/s10620-010-1316-0

Background The cag pathogenicity island (cag-PAI) is one of the major virulence factors of Helicobacter pylori, showing considerable geographic variation. Aim We investigated the prevalence of cagA, cagE, and cagT genes of cag-PAI and their association with proinflammatory IL-1B-511/-31/+3954 polymorphisms in Venezuelan chronic gastritis patients from a high-risk gastric cancer region. Methods Presence of cag-PAI genes and IL-1B polymorphisms in 121 biopsy specimens was evaluated by polymerase chain reaction (PCR) and PCR-restriction fragment length polymorphism (RFLP), respectively. Results cagA ⁺ and triple-positive (cagAET ⁺) strains were detected in 79.3% and 70.2% of patients, respectively. We found that infection with cagA ⁺ and cagAET ⁺ strains was associated (P < 0.05) with hosts harboring both IL-1B +3954C allele and IL-1B-511T/-31C/+3954C haplotype (TCC ⁺). The frequency of gastric atrophy was significantly higher (P < 0.020) among cagAET ⁺/IL-1B-TCC ⁺ combined genotype carriers. Conclusion Carriage of IL-1B +3954C allele and IL-1B-TCC ⁺ haplotype could favor colonization of bacterial cagAET ⁺ strains, and the combination of these bacterial and host haplotypes could play a synergistic role in development of premalignant gastric lesions. This work contributes to understanding of the complex interaction between H. pylori virulence factors and cytokine genotypes involved in gastrointestinal diseases.